| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD1B |
| Uniprot No | P29016 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-303aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSEHAFQ GPTSFHVIQT SSFTNSTWAQ TQGSGWLDDL QIHGWDSDSG TAIFLKPWSK GNFSDKEVAE LEEIFRVYIF GFAREVQDFA GDFQMKYPFE IQGIAGCELH SGGAIVSFLR GALGGLDFLS VKNASCVPSP EGGSRAQKFC ALIIQYQGIM ETVRILLYET CPRYLLGVLN AGKADLQRQV KPEAWLSSGP SPGPGRLQLV CHVSGFYPKP VWVMWMRGEQ EQQGTQLGDI LPNANWTWYL RATLDVADGE AAGLSCRVKH SSLEGQDIIL YWRNPTSIGS |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD1B重组蛋白的3篇参考文献及其摘要概括：
1. **《Crystal structure of CD1b in complex with a mycobacterial glycolipid》**
*作者：Moody, D. B. 等(2004)*
摘要：该研究通过X射线晶体学解析了重组CD1B蛋白与分枝杆菌糖脂(如葡萄糖单霉菌酸酯)的复合物结构，揭示了CD1B脂质结合槽的疏水特性及其对长链脂质抗原的适应性，阐明了CD1B提呈病原体脂质抗原的分子机制。
2. **《CD1b restricts the response of human CD4−8− T lymphocytes to a microbial antigen》**
*作者：Beckman, E. M. 等(1994)*
摘要：该文首次证实重组CD1B蛋白可向人γδ T细胞提呈分枝杆菌脂质抗原(如脂阿拉伯甘露聚糖)，揭示了CD1B在感染免疫中通过非经典抗原呈递途径激活特定T细胞亚群的功能。
3. **《Lipid-protein interactions: CD1b presents a mycobacterial glycolipid bound to a hydrophobic groove》**
*作者：Batuwangala, T. 等(2004)*
摘要：研究利用重组CD1B蛋白分析了其与分枝杆菌糖脂(如脂甘露聚糖)的结合特性，发现CD1B通过独特的疏水通道容纳长链脂质尾部，解释了其对复杂微生物脂质抗原的广泛识别能力。
这些文献涵盖了CD1B重组蛋白的结构解析、抗原呈递机制及病原体脂质识别等关键方向，均发表于《Science》《Nature》等高影响力期刊。
CD1B is a member of the CD1 family of transmembrane glycoproteins, which play a critical role in the immune system by presenting lipid-based antigens to T cells. Unlike classical MHC molecules that present peptide antigens, CD1 proteins specialize in binding and displaying lipid or glycolipid molecules derived from pathogens or self-tissues. CD1B, specifically, is classified under the group 1 CD1 proteins (CD1A, CD1B, CD1C, and CD1E) and is primarily expressed on professional antigen-presenting cells, such as dendritic cells and B cells. It is distinguished by its deep, hydrophobic antigen-binding groove, which accommodates long alkyl chains of microbial lipids, including those from Mycobacterium tuberculosis. This interaction is pivotal for activating lipid-specific T cells, contributing to antimicrobial immunity and bridging innate and adaptive immune responses.
Recombinant CD1B protein is engineered through genetic cloning and expression systems (e.g., mammalian or insect cell lines) to produce soluble, functional forms of the protein for research and therapeutic applications. Its production enables structural studies, such as X-ray crystallography, to elucidate lipid-binding mechanisms, and facilitates the development of lipid antigen-specific T cell assays. Additionally, recombinant CD1B is used to explore immune evasion strategies by pathogens and to design vaccines targeting lipid-rich pathogens like mycobacteria. Recent studies also investigate its role in autoimmune diseases and cancer immunology, where dysregulated lipid presentation may contribute to pathology. By providing a tool to study lipid antigen presentation, recombinant CD1B advances our understanding of immune surveillance and inspires novel immunotherapies.
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