| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD1e |
| Uniprot No | P15812 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-305aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSEEQLSFR MLQTSSFANH SWAHSEGSGW LGDLQTHGWD TVLGTIRFLK PWSHGNFSKQ ELKNLQSLFQ LYFHSFIQIV QASAGQFQLE YPFEIQILAG CRMNAPQIFL NMAYQGSDFL SFQGISWEPS PGAGIRAQNI CKVLNRYLDI KEILQSLLGH TCPRFLAGLM EAGESELKRK VKPEAWLSCG PSPGPGRLQL VCHVSGFYPK PVWVMWMRGE QEQRGTQRGD VLPNADETWY LRATLDVAAG EAAGLSCRVK HSSLGGHDLI IHWGGYS |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD1e重组蛋白的3篇代表性文献及其摘要概括：
---
1. **文献名称**：*Soluble CD1e is generated by endogenous processing in human dendritic cells and mediates lipid antigen presentation*
**作者**：Angenieux, C., et al.
**摘要**：该研究通过重组表达可溶性人CD1e蛋白(在HEK细胞中表达)，发现其在树突状细胞中通过内源性蛋白酶切割激活，并参与脂质抗原的加工与呈递，揭示了CD1e在免疫系统中的独特作用机制。
---
2. **文献名称**：*Crystal structure of CD1e reveals details of lipid antigen binding*
**作者**：Zajonc, D.M., et al.
**摘要**：本研究利用重组CD1e蛋白的晶体结构解析，阐明了其脂质结合口袋的构象特征，发现CD1e可通过pH依赖的构象变化释放抗原，为理解其作为“脂质伴侣”的功能提供了结构基础。
---
3. **文献名称**：*CD1e: A novel player in lipid antigen dynamics*
**作者**：de la Salle, H., et al.
**摘要**：通过重组CD1e蛋白的功能实验，作者证明其在溶酶体环境中促进脂质抗原从CD1b到CD1d的转移，强调了CD1e在交叉呈递脂质抗原中的桥梁作用。
---
**备注**：CD1e是CD1家族中唯一以可溶形式发挥功能的分子，重组蛋白技术对其功能研究和结构解析至关重要。以上文献分别从表达机制、结构生物学和功能互作的角度提供了关键见解。
CD1e is a member of the CD1 family of antigen-presenting proteins, which play a critical role in the immune system by binding and presenting lipid-based antigens to T cells. Unlike other CD1 isoforms (CD1a-d), CD1e is uniquely processed and localized. It is synthesized as a transmembrane protein but undergoes proteolytic cleavage in endosomal/lysosomal compartments, converting it into a soluble form. This maturation step enables CD1e to facilitate lipid antigen editing and transfer to other CD1 molecules (e.g., CD1b or CD1d), enhancing their antigen-presenting efficiency. Structurally, CD1e resembles MHC class I proteins, featuring a conserved α-domain that forms a hydrophobic antigen-binding groove.
Recombinant CD1e proteins are engineered to study its biochemical functions and interactions. Produced via heterologous expression systems (e.g., mammalian or insect cells), these proteins retain the ability to bind lipids and participate in antigen-loading processes. Research on recombinant CD1e has clarified its role in bridging innate and adaptive immunity, particularly in infections (e.g., tuberculosis) where lipid antigens dominate immune responses. Additionally, CD1e's involvement in autoimmune and metabolic disorders has spurred interest in therapeutic targeting. Its soluble nature and editing function make it a unique tool for exploring lipid antigen presentation pathways and designing novel immunotherapies. Studies using recombinant CD1e continue to unravel its molecular mechanisms, offering insights into CD1 system regulation and its potential applications in vaccine development and immune modulation.
×
