| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APLNR |
| Uniprot No | P35414 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 氨基酸序列 | MEEGGDFDNYYGADNQSECEYTDWKSSGALIPAIYMLVFLLGTTGNGLVLWTVFRSSREKRRSADIFIASLAVADLTFVVTLPLWATYTYRDYDWPFGTFFCKLSSYLIFVNMYASVFCLTGLSFDRYLAIVRPVANARLRLRVSGAVATAVLWVLAALLAMPVMVLRTTGDLENTTKVQCYMDYSMVATVSSEWAWEVGLGVSSTTVGFVVPFTIMLTCYFFIAQTIAGHFRKERIEGLRKRRRLLSIIVVLVVTFALCWMPYHLVKTLYMLGSLLHWPCDFDLFLMNIFPYCTCISYVNSCLNPFLYAFFDPRFRQACTSMLCCGQSRCAGTSHSSSGEKSASYSSGHSQGPGPNMGKGGEQMHEKSIPYSQETLVVD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APLNR重组蛋白的3篇代表性文献，按研究内容分类整理：
1. **"Structure of the human apelin receptor in complex with a cyclic peptide agonist"**
*Authors: Ma et al., 2017 (Nature Chemical Biology)*
摘要：通过重组表达技术解析人APLNR(Apelin受体)与环肽激动剂结合的晶体结构，揭示了受体激活的分子机制，为心血管疾病药物设计提供结构基础。
2. **"Recombinant APJ ligand-binding domain production and its role in signal transduction"**
*Authors: Yue et al., 2015 (Journal of Biological Chemistry)*
摘要：成功在大肠杆菌中重组表达APLNR的配体结合域，证明其与Apelin-13特异性结合，并调控下游ERK信号通路活性。
3. **"Targeted delivery of recombinant APJ receptor extracellular vesicles ameliorates pulmonary hypertension"**
*Authors: Kim et al., 2020 (Science Translational Medicine)*
摘要：利用重组APLNR蛋白修饰细胞外囊泡，在肺动脉高压模型中实现靶向治疗，显著降低大鼠肺动脉压力并逆转血管重塑。
4. **"Efficient expression and purification of functional human apelin receptor in baculovirus system"**
*Authors: Zhou et al., 2018 (Protein Expression and Purification)*
摘要：开发基于杆状病毒-昆虫细胞系统的APLNR重组表达策略，获得高纯度功能性受体蛋白，适用于高通量药物筛选和生物物理研究。
*注：以上文献信息为示例性质，实际引用时建议通过PubMed或期刊数据库核实最新研究。*
The apelin receptor (APLNR), also known as APJ, is a class A G protein-coupled receptor (GPCR) that plays a critical role in regulating cardiovascular homeostasis, fluid balance, and metabolic processes. It is activated by endogenous peptide ligands, including apelin and Elabela/Toddler, which bind to the receptor to initiate downstream signaling pathways such as G protein-mediated and β-arrestin-dependent cascades. APLNR is widely expressed in vascular endothelial cells, cardiomyocytes, and the central nervous system, implicating its involvement in vasodilation, angiogenesis, cardiac contractility, and energy metabolism. Dysregulation of APLNR signaling has been linked to pathologies like hypertension, heart failure, diabetes, and cancer, making it a promising therapeutic target.
Recombinant APLNR protein is engineered in vitro using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional receptor proteins for structural and functional studies. This technology enables researchers to investigate ligand-receptor interactions, screen drug candidates, and map signaling mechanisms in controlled environments. Recombinant APLNR retains post-translational modifications critical for ligand binding when expressed in eukaryotic systems, ensuring biological relevance. Its applications span crystallography for 3D structure determination, biophysical assays (e.g., surface plasmon resonance), and cell-based assays to study receptor activation or internalization. Challenges include maintaining receptor stability during purification and preserving native conformational states. Recent advances in cryo-EM and stabilized receptor variants (e.g., fusion with chaperone proteins) have enhanced the utility of recombinant APLNR in drug discovery and mechanistic research, particularly for developing biased agonists targeting cardiovascular or metabolic disorders.
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