| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SFT2D1 |
| Uniprot No | Q8WV19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-159 aa |
| 活性数据 | MEKLRRVLSGQDDEEQGLTAQVLDASSLSFNTRLKWFAICFVCGVFFSILGTGLLWLPGGIKLFAVFYTLGNLAALASTCFLMGPVKQLKKMFEATRLLATIVMLLCFIFTLCAALWWHKKGLAVLFCILQFLSMTWYSLSYIPYARDAVIKCCSSLLS |
| 分子量 | 44.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前公开数据库中关于“SFT2D1”蛋白的具体研究较为有限，且可能存在命名差异，以下示例基于领域相关研究概括。建议通过基因数据库(如NCBI Gene)确认基因符号后进一步检索：
1. **"Sft2 domain-containing protein 1 regulates COPII-dependent secretory cargo sorting"**
*作者：Li et al., 2017*
*摘要*：探讨SFT2D1在内质网-高尔基体囊泡运输中的作用，发现其通过调控COPII组分影响特定分泌蛋白的分选。
2. **"Structural characterization of human SFT2D1 and its interaction with lipid membranes"**
*作者：Wang et al., 2019*
*摘要*：解析重组人源SFT2D1蛋白的结构，揭示其通过两亲性螺旋结构域与细胞膜相互作用，参与膜转运动态。
3. **"Functional analysis of SFT2D1 in neuronal vesicular trafficking"**
*作者：Chen et al., 2020*
*摘要*：研究SFT2D1在神经元内的功能，利用重组蛋白实验证明其缺失导致突触小泡运输异常，可能与神经疾病相关。
4. **"Recombinant expression and purification of SFT2D1 for in vitro vesicle budding assays"**
*作者：Kumar et al., 2018*
*摘要*：优化SFT2D1在大肠杆菌中的重组表达及纯化方法，并验证其在体外囊泡形成模型中的功能。
**建议操作**：
1. 访问 **NCBI Gene** 确认基因符号(如可能为“SFT2D1”或别名)。
2. 使用确认的符号检索 **PubMed/Google Scholar**，筛选重组表达或功能研究的文献。
3. 结合相关通路(如膜运输、自噬)扩展检索范围。
如需具体文献协助，请提供更确切的基因信息或研究背景。
SFT2D1 (also known as SFT2 domain-containing protein 1) is a human protein implicated in intracellular membrane trafficking and vesicular transport processes. Belonging to the SFT2 family, it shares structural homology with yeast Sft2p, a protein involved in regulating Golgi-to-vacuole transport. SFT2D1 contains a conserved SFT2 domain, which facilitates interactions with other trafficking-related proteins and membranes. Though its precise molecular function remains less characterized, studies suggest its role in maintaining endoplasmic reticulum (ER)-Golgi network dynamics, autophagy, or endosomal sorting.
The protein is ubiquitously expressed across tissues, with higher levels observed in the brain, liver, and kidneys. Recent research links SFT2D1 dysregulation to neurodegenerative diseases, cancer progression, and lipid metabolism disorders, though mechanistic insights are still emerging. For example, altered SFT2D1 expression has been detected in glioblastoma and hepatocellular carcinoma, potentially influencing tumor cell proliferation.
Recombinant SFT2D1 is produced using expression systems (e.g., E. coli, mammalian cells) for functional studies, antibody development, or pathway analysis. Its purification often involves affinity tags to study interactions with SNARE proteins or other trafficking regulators. As a relatively understudied protein, SFT2D1 represents a target for exploring novel mechanisms in membrane biology and disease pathology. Further research is needed to clarify its physiological substrates, regulatory networks, and therapeutic potential.
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