| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGCE |
| Uniprot No | O43556 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-437 aa |
| 活性数据 | MQLPRWWELGDPCAWTGQGRGTRRMSPATTGTFLLTVYSIFSKVHSDRNVYPSAGVLFVHVLEREYFKGEFPPYPKPGEISNDPITFNTNLMGYPDRPGWLRYIQRTPYSDGVLYGSPTAENVGKPTIIEITAYNRRTFETARHNLIINIMSAEDFPLPYQAEFFIKNMNVEEMLASEVLGDFLGAVKNVWQPERLNAINITSALDRGGRVPLPINDLKEGVYVMVGADVPFSSCLREVENPQNQLRCSQEMEPVITCDKKFRTQFYIDWCKISLVDKTKQVSTYQEVIRGEGILPDGGEYKPPSDSLKSRDYYTDFLITLAVPSAVALVLFLILAYIMCCRREGVEKRNMQTPDIQLVHHSAIQKSTKELRDMSKNREIAWPLSTLPVFHPVTGEIIPPLHTDNYDSTNMPLMQTQQNLPHQTQIPQQQTTGKWYP |
| 分子量 | 76.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SGCE蛋白的3篇示例参考文献，格式为虚构示例，供参考：
1. **文献名称**: "Recombinant Expression and Functional Analysis of Human SGCE in a Mammalian Cell System"
**作者**: Thompson, J.R. et al.
**摘要**: 该研究在HEK293细胞中成功表达了重组人SGCE蛋白，并通过免疫共沉淀验证了其与β-肌聚糖的相互作用，为研究SGCE突变导致的肌阵挛-肌张力障碍综合征提供分子基础。
2. **文献名称**: "Structural Characterization of Recombinant Human SGCE Using Cryo-Electron Microscopy"
**作者**: Suzuki, A. & Gomez, C.M.
**摘要**: 通过昆虫细胞系统表达并纯化SGCE蛋白，利用冷冻电镜解析了其三维结构，揭示了突变位点如何破坏蛋白稳定性，为疾病机制提供结构生物学证据。
3. **文献名称**: "Development of a Recombinant SGCE-Based Diagnostic Assay for Myoclonus-Dystonia Autoantibodies"
**作者**: Wang, L. et al.
**摘要**: 研究团队基于重组SGCE蛋白建立了一种新型ELISA检测方法，用于筛查患者血清中的自身抗体，显著提升了肌张力障碍相关疾病的诊断灵敏度。
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**备注**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索关键词“recombinant SGCE protein”或“SGCE expression”获取真实文献。
Sarcoglycan epsilon (SGCE) is a member of the sarcoglycan family, a subgroup of transmembrane proteins within the dystrophin-glycoprotein complex (DGC) that stabilizes cell membranes in muscle and non-muscle tissues. Structurally, SGCE contains a single transmembrane domain, intracellular N- and C-termini, and extracellular cysteine-rich regions critical for interactions with other DGC components. It plays a role in maintaining mechanical integrity during muscle contraction and cellular stress. Unlike α-, β-, γ-, and δ-sarcoglycans linked to limb-girdle muscular dystrophies, SGCE mutations are primarily associated with myoclonus-dystonia syndrome (DYT11), an autosomal dominant movement disorder characterized by rapid muscle jerks and dystonic postures.
Recombinant human SGCE protein, produced via mammalian or insect expression systems, retains post-translational modifications (e.g., glycosylation) to mimic native functionality. It serves as a vital tool for studying SGCE’s interactions within the DGC, elucidating pathomechanisms of DYT11. and screening therapeutic candidates. Research also explores its potential in gene therapy or protein replacement strategies to restore DGC stability in SGCE-deficient cells. Beyond neurology, SGCE’s involvement in cancer cell adhesion and signaling pathways highlights its broader biomedical relevance.
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