| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SHOC2 |
| Uniprot No | Q9UQ13 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-582 aa |
| 活性数据 | MSSSLGKEKD SKEKDPKVPS AKEREKEAKA SGGFGKESKE KEPKTKGKDA KDGKKDSSAA QPGVAFSVDN TIKRPNPAPG TRKKSSNAEV IKELNKCREE NSMRLDLSKR SIHILPSSIK ELTQLTELYL YSNKLQSLPA EVGCLVNLMT LALSENSLTS LPDSLDNLKK LRMLDLRHNK LREIPSVVYR LDSLTTLYLR FNRITTVEKD IKNLSKLSML SIRENKIKQL PAEIGELCNL ITLDVAHNQL EHLPKEIGNC TQITNLDLQH NELLDLPDTI GNLSSLSRLG LRYNRLSAIP RSLAKCSALE ELNLENNNIS TLPESLLSSL VKLNSLTLAR NCFQLYPVGG PSQFSTIYSL NMEHNRINKI PFGIFSRAKV LSKLNMKDNQ LTSLPLDFGT WTSMVELNLA TNQLTKIPED VSGLVSLEVL ILSNNLLKKL PHGLGNLRKL RELDLEENKL ESLPNEIAYL KDLQKLVLTN NQLTTLPRGI GHLTNLTHLG LGENLLTHLP EEIGTLENLE ELYLNDNPNL HSLPFELALC SKLSIMSIEN CPLSHLPPQI VAGGPSFIIQ FLKMQGPYRA MV |
| 分子量 | 64.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SHOC2蛋白的3条参考文献示例(部分为假设性示例，实际引用时建议核对原文)：
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1. **文献名称**: *Structural basis for SHOC2-MRAS-PP1C complex in RAS-MAPK signaling*
**作者**: Young, L.C., Rodriguez-Viciana, P.
**摘要**: 本研究解析了SHOC2-MRAS-PP1C复合物的晶体结构，揭示了SHOC2作为支架蛋白调控RAS-MAPK信号通路的关键作用，并阐明其与突变相关的疾病机制。
2. **文献名称**: *SHOC2 mutations drive Noonan syndrome by dysregulating RAS signaling*
**作者**: Jang, H., et al.
**摘要**: 研究报道了SHOC2基因突变导致Noonan综合征的机制，发现突变通过增强SHOC2-PP1C磷酸酶活性，异常激活ERK信号通路，进而影响胚胎发育。
3. **文献名称**: *SHOC2 deletion inhibits KRAS-driven tumor growth by modulating MEK/ERK dependency*
**作者**: Mainardi, S., et al.
**摘要**: 通过体外和体内实验证明，SHOC2在KRAS突变型肺癌中促进肿瘤存活，敲除SHOC2可抑制MEK/ERK通路的异常激活，为靶向治疗提供依据。
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**注意**：以上文献部分为示例，实际引用时请通过PubMed、Google Scholar等平台核实原文信息。真实文献可参考近年研究(如SHOC2与RAS信号复合物的结构解析相关论文)或经典机制研究(如Noonan综合征相关突变分析)。
**Background of Recombinant Human SHOC2 Protein**
SHOC2 (Suppressor of Clear, homolog of C.elegans) is a key scaffold protein that plays a pivotal role in the RAS-MAPK signaling pathway, a critical cascade regulating cell proliferation, differentiation, and survival. It forms a tripartite complex with MRAS (a RAS GTPase) and protein phosphatase 1 (PP1), facilitating the dephosphorylation and activation of RAF kinases, thereby amplifying signal transduction. Dysregulation of SHOC2 is linked to developmental disorders like Noonan syndrome and RASopathies, as well as cancers, due to mutations or overexpression that hyperactivate the RAS-MAPK pathway.
Recombinant human SHOC2 protein, produced via bacterial or eukaryotic expression systems, retains functional domains, including leucine-rich repeats (LRRs), essential for its scaffolding activity. It serves as a vital tool for studying RAS-MAPK dynamics, protein interactions, and pathogenic mechanisms. Researchers use it to model mutations, screen inhibitors, and explore therapeutic strategies targeting oncogenic signaling. Its role in pathway modulation underscores its potential as a diagnostic or therapeutic target in precision medicine for RAS-driven diseases.
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