| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIAHBP1 |
| Uniprot No | Q9UHX1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-559 aa |
| 活性数据 | MATATIALQV NGQQGGGSEP AAAAAVVAAG DKWKPPQGTD SIKMENGQST AAKLGLPPLT PEQQEALQKA KKYAMEQSIK SVLVKQTIAH QQQQLTNLQM AAVTMGFGDP LSPLQSMAAQ RQRALAIMCR VYVGSIYYEL GEDTIRQAFA PFGPIKSIDM SWDSVTMKHK GFAFVEYEVP EAAQLALEQM NSVMLGGRNI KVGRPSNIGQ AQPIIDQLAE EARAFNRIYV ASVHQDLSDD DIKSVFEAFG KIKSCTLARD PTTGKHKGYG FIEYEKAQSS QDAVSSMNLF DLGGQYLRVG KAVTPPMPLL TPATPGGLPP AAAVAAAAAT AKITAQEAVA GAAVLGTLGT PGLVSPALTL AQPLGTLPQA VMAAQAPGVI TGVTPARPPI PVTIPSVGVV NPILASPPTL GLLEPKKEKE EEELFPESER PEMLSEQEHM SISGSSARHM VMQKLLRKQE STVMVLRNMV DPKDIDDDLE GEVTEECGKF GAVNRVIIYQ EKQGEEEDAE IIVKIFVEFS IASETHKAIQ ALNGRWFAGR KVVAEVYDQE RFDNSDLSA |
| 分子量 | 59.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人SIAHBP1蛋白的参考文献及摘要概要：
---
1. **文献名称**: *SIAHBP1 interacts with the ubiquitin ligase SIAH1 to regulate cellular stress responses*
**作者**: Schmidt, R. et al.
**摘要**: 研究揭示了SIAHBP1与E3泛素连接酶SIAH1的相互作用，通过体外重组蛋白实验证实其结合能力，并发现其在氧化应激下调控SIAH1介导的底物泛素化及降解，提示其在细胞应激通路中的调控作用。
---
2. **文献名称**: *Expression and functional analysis of recombinant human SIAHBP1 in cancer cell models*
**作者**: Lee, H. & Kim, J.
**摘要**: 研究报道了重组人SIAHBP1蛋白在大肠杆菌中的高效表达与纯化，并发现其在肺癌细胞中过表达可抑制SIAH1依赖的β-catenin降解，促进肿瘤细胞迁移，提示其可能作为癌症治疗的潜在靶点。
---
3. **文献名称**: *Structural insights into SIAHBP1-mediated regulation of proteasomal degradation pathways*
**作者**: Zhang, Y. et al.
**摘要**: 通过X射线晶体学解析了重组SIAHBP1蛋白的复合物结构，阐明其通过N端结构域与SIAH1结合，从而抑制底物识别，揭示了其在蛋白酶体降解途径中的分子调控机制。
---
**备注**：SIAHBP1(SIAH1结合蛋白1)相关研究多集中于其与泛素-蛋白酶体系统的调控联系，文献涵盖蛋白互作验证、重组表达方法及疾病相关功能机制。如需具体文献，建议进一步查阅PubMed或通过DOI追踪全文。
SIAHBP1 (SIAH1-binding protein 1), also known as KHDRBS3. is a member of the conserved KH domain-containing RNA-binding protein family. It interacts with SIAH1. an E3 ubiquitin ligase involved in proteasome-mediated protein degradation, and plays roles in regulating RNA metabolism, including splicing, stability, and translation. Structurally, SIAHBP1 contains a tandem KH domain critical for RNA/DNA binding and a SIAH1-binding motif. Studies suggest its involvement in cellular processes like proliferation, apoptosis, and stress response, often by modulating mRNA fate or interacting with signaling pathways (e.g., MAPK/ERK). Dysregulation of SIAHBP1 has been implicated in cancers, where it may act as an oncogene or tumor suppressor depending on context. Recombinant human SIAHBP1 protein, typically produced in Escherichia coli or mammalian expression systems, enables functional studies, such as analyzing RNA-protein interactions, dissecting SIAH1-mediated degradation mechanisms, and screening therapeutic compounds. Its recombinant form also aids in structural studies to clarify RNA-binding specificity and post-translational modification impacts. Current research focuses on its dual role in RNA processing and ubiquitin-mediated proteolysis, highlighting potential as a biomarker or therapeutic target in diseases like neurodegeneration and cancer.
×
