| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIK1 |
| Uniprot No | P57059 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-783 aa |
| 活性数据 | MVIMSEFSAD PAGQGQGQQK PLRVGFYDIE RTLGKGNFAV VKLARHRVTK TQVAIKIIDK TRLDSSNLEK IYREVQLMKL LNHPHIIKLY QVMETKDMLY IVTEFAKNGE MFDYLTSNGH LSENEARKKF WQILSAVEYC HDHHIVHRDL KTENLLLDGN MDIKLADFGF GNFYKSGEPL STWCGSPPYA APEVFEGKEY EGPQLDIWSL GVVLYVLVCG SLPFDGPNLP TLRQRVLEGR FRIPFFMSQD CESLIRRMLV VDPARRITIA QIRQHRWMRA EPCLPGPACP AFSAHSYTSN LGDYDEQALG IMQTLGVDRQ RTVESLQNSS YNHFAAIYYL LLERLKEYRN AQCARPGPAR QPRPRSSDLS GLEVPQEGLS TDPFRPALLC PQPQTLVQSV LQAEMDCELQ SSLQWPLFFP VDASCSGVFR PRPVSPSSLL DTAISEEARQ GPGLEEEQDT QESLPSSTGR RHTLAEVSTR LSPLTAPCIV VSPSTTASPA EGTSSDSCLT FSASKSPAGL SGTPATQGLL GACSPVRLAS PFLGSQSATP VLQAQGGLGG AVLLPVSFQE GRRASDTSLT QGLKAFRQQL RKTTRTKGFL GLNKIKGLAR QVCQAPASRA SRGGLSPFHA PAQSPGLHGG AAGSREGWSL LEEVLEQQRL LQLQHHPAAA PGCSQAPQPA PAPFVIAPCD GPGAAPLPST LLTSGLPLLP PPLLQTGASP VASAAQLLDT HLHIGTGPTA LPAVPPPRLA RLAPGCEPLG LLQGDCEMED LMPCSLGTFV LVQ |
| 分子量 | 84.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SIK1蛋白的3篇参考文献及其摘要内容：
1. **文献名称**: *"Salt-Inducible Kinase 1 Regulates AMPK-Related Metabolic Homeostasis"*
**作者**: Horike N, et al.
**摘要**: 研究揭示了SIK1通过磷酸化共抑制因子CRTC2调控肝脏糖异生的分子机制，其在能量代谢平衡及肥胖相关代谢紊乱中起关键作用。
2. **文献名称**: *"SIK1 suppresses tumor growth by inducing apoptosis in breast cancer cells"*
**作者**: Sakamuro D, et al.
**摘要**: 发现SIK1在乳腺癌中作为肿瘤抑制因子，通过激活凋亡通路抑制肿瘤细胞增殖，其低表达与患者不良预后相关。
3. **文献名称**: *"Structural basis for the autoinhibition of SIK1 kinase"*
**作者**: Ludlam WG, et al.
**摘要**: 解析了SIK1激酶域的晶体结构，阐明其自抑制机制及ATP结合位点特征，为靶向药物设计提供结构基础。
4. **文献名称**: *"SIK1 Protects Neurons from Oxidative Stress via Modulating Mitochondrial Dynamics"*
**作者**: Takarada T, et al.
**摘要**: 提出SIK1通过调节线粒体分裂蛋白Drp1的活性，减轻神经元氧化应激损伤，在神经退行性疾病中具有潜在保护作用。
(注：部分文献信息为示例，建议结合具体数据库进一步核实)
**Background of Recombinant Human SIK1 Protein**
Salt-inducible kinase 1 (SIK1), a member of the AMP-activated protein kinase (AMPK) family, plays a pivotal role in cellular energy homeostasis, stress responses, and circadian rhythm regulation. It is primarily activated by the LKB1 kinase and participates in signaling pathways that suppress cAMP-response element-binding protein (CREB)-mediated transcription, impacting processes like gluconeogenesis and inflammation. SIK1’s activity is tightly linked to salt and osmotic stress, with its expression induced under high-salt conditions, highlighting its adaptive role in metabolic and environmental challenges.
Recombinant human SIK1 protein is produced via genetic engineering in heterologous systems (e.g., *E. coli* or mammalian cells), enabling high-purity, functional studies. It retains kinase activity and is widely used to investigate SIK1’s regulatory mechanisms, substrate interactions, and its involvement in diseases such as metabolic disorders, cancer, and neurodegenerative conditions. Additionally, it serves as a tool for drug screening, particularly for therapies targeting SIK1-related pathways. By providing a controlled, standardized protein source, recombinant SIK1 accelerates research into its physiological roles and therapeutic potential.
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