| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLAIN2 |
| Uniprot No | Q9P270 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-581 aa |
| 活性数据 | MEDVNSNVNA DQEVRKLQEL VKKLEKQNEQ LRSRSGAVQG AGSLGPGSPV RAGASIPSSG AASPRGFPLG LSAKSGGGPG SGPRRTSSEE LRDATSLLAA GEGGLLDEVE PLRPDELERL SGWEEEEESW LYSSPKKKLT PMQKSVSPLV WCRQVLDYPS PDVECAKKSL IHKLDQTMSA LKRQNLYNNP FNSMSYTSPY SPNASSPYSS GFNSPSSTPV RPPIVKQLIL PGNSGNLKSS DRNPPLSPQS SIDSELSASE LDEDSIGSNY KLNDVTDVQI LARMQEESLR QEYAATTSRR SSGSSCNSTR RGTFSDQELD AQSLDDEDDN MHHAVYPAVN RFSPSPRNSP RPSPKQSPRN SPRSRSPARG IEYSRVSPQP MISRLQQPRL SLQGHPTDLQ TSNVKNEEKL RRSLPNLSRT SNTQVDSVKS SRSDSNFQVP NGGIPRMQPQ ASAIPSPGKF RSPAAPSPLA LRQPVKAFSN HGSGSPGSQE ITQLTQTTSS PGPPMVQSTV SANPPSNINS ATLTRPAGTT AMRSGLPRPS APSAGGIPVP RSKLAQPVRR SLPAPKTYGS MKDDSWKDGC Y |
| 分子量 | 62.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLAIN2蛋白的假设性参考文献示例(文献信息为模拟，建议通过学术数据库核实)：
1. **Title**: *"SLAIN2 links microtubule plus-end tracking proteins and regulates neuronal polarization"*
**Authors**: Homma, N., Takei, Y., & Hirokawa, N. (2014)
**Summary**：研究发现SLAIN2与蛋白激酶CK2相互作用，通过稳定微管正端动态调控神经元轴突极化，缺失SLAIN2会导致神经元迁移障碍。
2. **Title**: *"Structural insights into SLAIN2-mediated microtubule anchoring"*
**Authors**: Schoborg, T.A., & Rusan, N.M. (2016)
**Summary**：通过冷冻电镜解析SLAIN2结构，揭示其C端结构域结合微管并与CLASP蛋白互作，调控微管锚定至细胞边缘的机制。
3. **Title**: *"SLAIN2 depletion impairs cancer cell migration via RhoA/ROCK signaling suppression"*
**Authors**: Niu, H., Zhang, L., & Zheng, Y. (2018)
**Summary**：在乳腺癌模型中发现，SLAIN2通过调控RhoA活性促进细胞迁移和侵袭，其缺失导致细胞骨架重塑抑制和转移减少。
4. **Title**: *"Phosphorylation of SLAIN2 by CK2 modulates endosomal trafficking"*
**Authors**: Dephoure, N., Zhou, C., & Harper, J.W. (2015)
**Summary**：系统性分析SLAIN2的磷酸化修饰，发现CK2介导的磷酸化位点对SLAIN2结合内体并调控囊泡运输的功能至关重要。
**注**：以上文献为假设示例，实际研究请查询PubMed、Web of Science等平台，使用关键词“SLAIN2”或“SLAIN motif family”获取最新成果。
SLAIN2 (SLAIN motif containing 2) is a human protein implicated in intracellular trafficking and neuronal development. It contains a conserved C-terminal SLAIN domain and a N-terminal intrinsically disordered region, enabling interactions with various partners, particularly those involved in microtubule dynamics. Studies suggest SLAIN2 functions as a scaffolding protein, modulating dynein/dynactin-mediated retrograde transport by linking motor complexes to cargo adaptors. It is highly expressed in the brain and associates with endosomes, suggesting roles in neuronal polarity, dendritic branching, and synaptic organization.
Recombinant human SLAIN2 protein, typically produced in bacterial or mammalian expression systems, serves as a crucial tool for structural and functional studies. Researchers utilize it to investigate binding partners (e.g., CLIP-170. dynactin subunits) and phosphorylation events regulated by kinases like MARK2. which influence microtubule interaction dynamics. Mutations or dysregulation of SLAIN2 have been tentatively linked to neurodevelopmental disorders, motivating ongoing research into its pathological mechanisms. Recent work also explores its potential involvement in cancer cell motility. Recombinant variants (e.g., truncated forms, fluorescently tagged) enable live-cell imaging and high-resolution mapping of transport pathways, advancing understanding of intracellular logistics in health and disease.
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