| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SKI |
| Uniprot No | P12755 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-728 aa |
| 活性数据 | MEAAAGGRGC FQPHPGLQKT LEQFHLSSMS SLGGPAAFSA RWAQEAYKKE SAKEAGAAAV PAPVPAATEP PPVLHLPAIQ PPPPVLPGPF FMPSDRSTER CETVLEGETI SCFVVGGEKR LCLPQILNSV LRDFSLQQIN AVCDELHIYC SRCTADQLEI LKVMGILPFS APSCGLITKT DAERLCNALL YGGAYPPPCK KELAASLALG LELSERSVRV YHECFGKCKG LLVPELYSSP SAACIQCLDC RLMYPPHKFV VHSHKALENR TCHWGFDSAN WRAYILLSQD YTGKEEQARL GRCLDDVKEK FDYGNKYKRR VPRVSSEPPA SIRPKTDDTS SQSPAPSEKD KPSSWLRTLA GSSNKSLGCV HPRQRLSAFR PWSPAVSASE KELSPHLPAL IRDSFYSYKS FETAVAPNVA LAPPAQQKVV SSPPCAAAVS RAPEPLATCT QPRKRKLTVD TPGAPETLAP VAAPEEDKDS EAEVEVESRE EFTSSLSSLS SPSFTSSSSA KDLGSPGARA LPSAVPDAAA PADAPSGLEA ELEHLRQALE GGLDTKEAKE KFLHEVVKMR VKQEEKLSAA LQAKRSLHQE LEFLRVAKKE KLREATEAKR NLRKEIERLR AENEKKMKEA NESRLRLKRE LEQARQARVC DKGCEAGRLR AKYSAQIEDL QVKLQHAEAD REQLRADLLR EREAREHLEK VVKELQEQLW PRARPEAAGS EGAAELEP |
| 分子量 | 80.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SKI蛋白的3篇代表性参考文献(基于模拟信息整理)：
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1. **文献名称**: *Recombinant Human SKI Protein Inhibits TGF-β Signaling and Attenuates Fibrosis in Experimental Models*
**作者**: Chen L, Wang Y, et al.
**摘要**: 本研究通过大肠杆菌系统表达了重组人SKI蛋白，并验证其抑制TGF-β信号通路的能力。体外实验显示，SKI蛋白通过结合Smad蛋白阻断TGF-β介导的纤维化基因表达;小鼠肺纤维化模型中，SKI显著降低胶原沉积，提示其在抗纤维化治疗中的潜力。
2. **文献名称**: *Structural and Functional Characterization of Recombinant SKI Protein in Cancer Cell Invasion*
**作者**: Gupta S, Patel RK, et al.
**摘要**: 文章解析了重组SKI蛋白的结构域功能，发现其N端结构域对抑制TGF-β诱导的肿瘤细胞迁移至关重要。在乳腺癌细胞系中，SKI过表达抑制上皮-间质转化(EMT)，降低细胞侵袭能力，表明其作为肿瘤转移治疗靶点的可能性。
3. **文献名称**: *SKI Enhances Reprogramming Efficiency by Modulating Mesenchymal-to-Epithelial Transition*
**作者**: Kim H, Lee J, et al.
**摘要**: 该研究发现重组SKI蛋白通过调控MET(间充质-上皮转化)促进体细胞重编程为诱导多能干细胞(iPSCs)。机制研究表明，SKI通过抑制TGF-β/Smad3信号通路，上调上皮标志物E-cadherin，为优化干细胞制备提供了新策略。
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注：以上文献及内容为模拟示例，实际研究中请根据具体论文内容调整。建议通过PubMed或Web of Science以“recombinant SKI protein”“TGF-beta signaling”等关键词检索最新文献。
Recombinant human SKI protein is a biologically engineered form of the SKI (Sarcoma amplified **SKI**) proto-oncogene-encoded protein, initially identified for its role in cellular transformation and tumorigenesis. Structurally, SKI is a multidomain protein containing conserved regions like the Ski/Sno homology domain, which mediates interactions with transcriptional regulators such as Smad proteins. Functionally, SKI acts as a transcriptional co-repressor, predominantly modulating the TGF-β (transforming growth factor-beta) signaling pathway. By binding to Smad complexes, SKI inhibits TGF-β-mediated transcriptional activation, thereby regulating processes like cell proliferation, differentiation, apoptosis, and epithelial-mesenchymal transition (EMT).
SKI’s dual role in cancer—acting as both an oncogene and tumor suppressor depending on context—has sparked significant research interest. Overexpression of SKI is linked to tumor progression in melanoma and sarcoma, while its downregulation correlates with metastasis in other cancers. Beyond oncology, SKI is implicated in fibrosis, developmental disorders, and tissue repair. Recombinant SKI protein, produced via bacterial or mammalian expression systems, is widely utilized to study these mechanisms. It enables researchers to dissect SKI’s interactions with signaling molecules, screen therapeutic compounds, and explore its potential in regenerative medicine.
Ongoing studies focus on SKI-targeted therapies, including small-molecule inhibitors or gene-editing approaches, to modulate its activity in diseases driven by TGF-β dysregulation. This makes recombinant SKI a critical tool in both basic research and translational applications.
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