| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIX4 |
| Uniprot No | Q9UIU6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-781 aa |
| 活性数据 | SSSSPTGQI ASAADIKQEN GMESASEGQE AHREVAGGAA VGLSPPAPAP FPLEPGDAAT AAARVSGEEG AVAAAAAGAA ADQVQLHSEL LGRHHHAAAA AAQTPLAFSP DHVACVCEAL QQGGNLDRLA RFLWSLPQSD LLRGNESLLK ARALVAFHQG IYPELYSILE SHSFESANHP LLQQLWYKAR YTEAERARGR PLGAVDKYRL RRKFPLPRTI WDGEETVYCF KEKSRNALKE LYKQNRYPSP AEKRHLAKIT GLSLTQVSNW FKNRRQRDRN PSETQSKSES DGNPSTEDES SKGHEDLSPH PLSSSSDGIT NLSLSSHMEP VYMQQIGNAK ISLSSSGVLL NGSLVPASTS PVFLNGNSFI QGPSGVILNG LNVGNTQAVA LNPPKMSSNI VSNGISMTDI LGSTSQDVKE FKVLQSSANS ATTTSYSPSV PVSFPGLIPS TEVKREGIQT VASQDGGSVV TFTTPVQINQ YGIVQIPNSG ANSQFLNGSI GFSPLQLPPV SVAASQGNIS VSSSTSDGST FTSESTTVQQ GKVFLSSLAP SAVVYTVPNT GQTIGSVKQE GLERSLVFSQ LMPVNQNAQV NANLSSENIS GSGLHPLASS LVNVSPTHNF SLSPSTLLNP TELNRDIADS QPMSAPVASK STVTSVSNTN YATLQNCSLI TGQDLLSVPM TQAALGEIVP TAEDQVGHPS PAVHQDFVQE HRLVLQSVAN MKENFLSNSE SKATSSLMML DSKSKYVLDG MVDTVCEDLE TDKKELAKLQ TVQLDEDMQD L |
| 分子量 | 82.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SIX4蛋白的3篇参考文献示例(文献信息及摘要基于领域研究趋势综合描述，仅供示例参考)：
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1. **文献名称**：*"Recombinant Human SIX4 Enhances Mesenchymal Stem Cell Differentiation via BMP Signaling"*
**作者**：Li X, et al.
**摘要**：研究通过大肠杆菌表达系统成功纯化重组人SIX4蛋白，并发现其通过激活BMP-Smad通路促进间充质干细胞向成骨细胞分化，表明其在骨骼再生中的潜在应用。
2. **文献名称**：*"Structural Insights into SIX4-DNA Interaction Using Recombinant Protein Crystallography"*
**作者**：Wang Y, et al.
**摘要**：利用重组表达的人源SIX4蛋白进行晶体结构解析，揭示了其N端DNA结合结构域与靶基因启动子的特异性结合模式，为SIX4的转录调控机制提供结构基础。
3. **文献名称**：*"Recombinant SIX4 Protein Induces Tumor Angiogenesis via VEGF Upregulation in Hepatocellular Carcinoma"*
**作者**：Tanaka K, et al.
**摘要**：体外实验表明，重组SIX4蛋白可通过HIF-1α依赖途径上调VEGF表达，促进肝癌血管生成，提示其在肿瘤微环境中的促癌作用。
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**说明**：SIX4蛋白在发育调控和疾病中的研究相对较少，以上摘要基于相关领域(如干细胞分化、癌症机制)的常见研究逻辑推演，实际文献需通过数据库(如PubMed)以关键词“recombinant SIX4”检索核实。部分研究可能集中在SIX家族其他成员(如SIX1)，而针对SIX4的重组蛋白功能研究仍需进一步探索。
SIX4 (Sine oculis homeobox homolog 4) is a member of the SIX family of evolutionarily conserved transcription factors, characterized by a DNA-binding homeodomain and a SIX domain critical for protein-protein interactions. It plays pivotal roles during embryonic development, particularly in myogenesis, neurogenesis, and organogenesis. SIX4 regulates tissue-specific gene expression by interacting with Eya family co-factors, forming functional complexes that influence cell proliferation, differentiation, and survival. Studies in model organisms highlight its involvement in skeletal muscle formation, kidney development, and sensory organ patterning.
Dysregulation of SIX4 has been implicated in human diseases, including cancers such as rhabdomyosarcoma and hepatocellular carcinoma, where its aberrant expression correlates with tumor progression and metastasis. As a recombinant protein, SIX4 is typically produced in heterologous systems (e.g., E. coli or mammalian cells) for functional studies. Researchers utilize recombinant SIX4 to dissect its molecular interactions, transcriptional regulatory mechanisms, and potential therapeutic applications. Its role in regenerative medicine is also under exploration due to its capacity to modulate progenitor cell fate. Current research focuses on elucidating its context-dependent functions and validating its diagnostic or therapeutic relevance in pathological conditions.
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