| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIPA1 |
| Uniprot No | Q96FS4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 933-1042 aa |
| 活性数据 | SEIASTCNTILESLSREGQPIPESGDPKGTPKSDAEPEPGNLSEKVSHLESMLRKLQEDLQKEKADRAALEEEVRSLRHNNRRLQAESESAATRLLLASKQLGSPTADLA |
| 分子量 | 37.73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SIPA1蛋白的参考文献示例(注：部分信息为示例性虚构，建议通过学术数据库核实具体文献)：
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1. **文献名称**: "Recombinant Human SIPA1 Protein Enhances Metastatic Potential via Rap1 GTPase Activation"
**作者**: Zhang L, et al.
**摘要**: 本研究通过在大肠杆菌中表达并纯化重组人SIPA1蛋白，发现其通过激活Rap1 GTP酶活性促进肿瘤细胞的迁移和侵袭，揭示了SIPA1在癌症转移中的分子机制。
2. **文献名称**: "Functional Characterization of SIPA1 in Breast Cancer Progression Using Recombinant Protein Models"
**作者**: Tanaka K, et al.
**摘要**: 利用重组SIPA1蛋白进行体外实验，证明其通过调控β-catenin信号通路促进乳腺癌细胞增殖和上皮-间质转化(EMT)，提示其作为治疗靶点的潜力。
3. **文献名称**: "Purification and Structural Analysis of Recombinant SIPA1: Implications for GTPase Regulatory Function"
**作者**: Smith J, et al.
**摘要**: 报道了一种高效的昆虫细胞表达系统制备重组人SIPA1蛋白的方法，结合晶体学分析揭示了其GAP结构域与Rap1的结合模式，为靶向药物设计提供结构基础。
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**备注**：以上文献信息仅供示例参考，实际研究中请通过**PubMed**、**Web of Science**或**Google Scholar**以关键词“recombinant SIPA1 protein”“SIPA1 signaling”等检索最新文献。
**Background of Recombinant Human SIPA1 Protein**
Signal-Induced Proliferation-Associated 1 (SIPA1) is a GTPase-activating protein (GAP) belonging to the Rap GAP family, primarily recognized for its role in modulating intracellular signaling pathways linked to cell proliferation, adhesion, and migration. Encoded by the *SIPA1* gene located on human chromosome 11q13. it regulates Rap GTPases by accelerating their hydrolysis of GTP to GDP, thereby controlling Rap-mediated signal transduction. This activity influences integrin-dependent cell-matrix interactions and cytoskeletal reorganization, critical for cellular motility and invasive behavior.
Structurally, SIPA1 contains a conserved Rap-GAP domain and a PDZ-binding motif, facilitating interactions with proteins like β-catenin, which links it to pathways such as Wnt signaling. Research highlights its involvement in cancer progression, particularly metastasis. Elevated SIPA1 expression correlates with aggressive phenotypes in breast, prostate, and colorectal cancers, where it promotes invasiveness by enhancing extracellular matrix degradation and metastatic niche formation. Polymorphisms in *SIPA1* have also been associated with cancer susceptibility and metastasis efficiency in murine models.
Recombinant human SIPA1 protein is engineered for in vitro and in vivo studies to dissect its biochemical functions, signaling crosstalk, and therapeutic potential. It serves as a tool to explore metastasis mechanisms and screen inhibitors targeting its GAP activity. Understanding SIPA1's role may advance strategies to curb tumor dissemination or improve prognostic biomarkers.
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