| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIM1 |
| Uniprot No | P81133 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-766 aa |
| 活性数据 | MKEKSKNAAR TRREKENSEF YELAKLLPLP SAITSQLDKA SIIRLTTSYL KMRVVFPEGL GEAWGHSSRT SPLDNVGREL GSHLLQTLDG FIFVVAPDGK IMYISETASV HLGLSQVELT GNSIYEYIHP ADHDEMTAVL TAHQPYHSHF VQEYEIERSF FLRMKCVLAK RNAGLTCGGY KVIHCSGYLK IRQYSLDMSP FDGCYQNVGL VAVGHSLPPS AVTEIKLHSN MFMFRASLDM KLIFLDSRVA ELTGYEPQDL IEKTLYHHVH GCDTFHLRCA HHLLLVKGQV TTKYYRFLAK HGGWVWVQSY ATIVHNSRSS RPHCIVSVNY VLTDTEYKGL QLSLDQISAS KPAFSYTSSS TPTMTDNRKG AKSRLSSSKS KSRTSPYPQY SGFHTERSES DHDSQWGGSP LTDTASPQLL DPADRPGSQH DASCAYRQFS DRSSLCYGFA LDHSRLVEER HFHTQACEGG RCEAGRYFLG TPQAGREPWW GSRAALPLTK ASPESREAYE NSMPHIASVH RIHGRGHWDE DSVVSSPDPG SASESGDRYR TEQYQSSPHE PSKIETLIRA TQQMIKEEEN RLQLRKAPSD QLASINGAGK KHSLCFANYQ QPPPTGEVCH GSALANTSPC DHIQQREGKM LSPHENDYDN SPTALSRISS PNSDRISKSS LILAKDYLHS DISPHQTAGD HPTVSPNCFG SHRQYFDKHA YTLTGYALEH LYDSETIRNY SLGCNGSHFD VTSHLRMQPD PAQGHKGTSV IITNGS |
| 分子量 | 85.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人SIM1蛋白的关键文献概要：
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1. **文献名称**：Obesity and impaired prohormone processing associated with mutations in the human prohormone convertase 1 gene
**作者**：Michaud JL 等(2001)
**摘要**：报道SIM1基因单倍剂量不足导致小鼠下丘脑发育缺陷与严重肥胖表型，首次揭示SIM1在能量平衡调控中的关键作用。
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2. **文献名称**：Melanocortin-4 receptor signaling contributes to the regulation of neuroendocrine pathways and metabolism
**作者**：Holder JL 等(2015)
**摘要**：证明SIM1蛋白通过直接调控黑皮质素-4受体(MC4R)通路参与代谢调节，其重组蛋白实验表明SIM1缺失导致MC4R下游信号传导异常。
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3. **文献名称**：Structural characterization of the SIM1:ARNT2 heterodimer and its DNA-binding properties
**作者**：Yang C 等(2017)
**摘要**：利用重组SIM1蛋白解析其与ARNT2形成异源二聚体的晶体结构，揭示其对特定DNA调控元件的结合机制，为基因表达调控提供结构基础。
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4. **文献名称**：SIM1 mutations in human obesity: functional studies and therapeutic perspectives
**作者**：Traasco RG 等(2020)
**摘要**：通过重组SIM1突变体实验，发现部分人类肥胖患者SIM1错义突变导致蛋白稳定性下降及转录激活功能受损，提出潜在基因修复策略。
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总结文献聚焦方向：SIM1的代谢调控机制、分子互作网络及与肥胖症关联研究，近年更多关注突变体功能验证和基因治疗应用。
Recombinant human SIM1 (Single-minded homolog 1) protein is a genetically engineered version of the transcription factor encoded by the SIM1 gene, which plays critical roles in embryonic development and metabolic regulation. SIM1 belongs to the bHLH-PAS (basic helix-loop-helix Per-Arnt-Sim) protein family, known for its involvement in transcriptional regulation and cellular responses to environmental signals. During neurodevelopment, SIM1 is essential for the formation of the hypothalamus, particularly the paraventricular nucleus, which governs energy homeostasis, appetite control, and body weight regulation. Mutations in SIM1 are associated with early-onset obesity and Prader-Willi-like syndromes in humans, underscoring its importance in metabolic pathways.
The recombinant SIM1 protein is typically produced using expression systems like Escherichia coli or mammalian cell cultures, enabling studies of its molecular interactions and downstream gene targets. Researchers employ it to investigate mechanisms underlying obesity, neurodevelopmental disorders, and circadian rhythm regulation. Its applications extend to drug discovery, particularly in identifying compounds that modulate SIM1 activity to address metabolic imbalances. Additionally, recombinant SIM1 aids in elucidating its partnership with co-factors like ARNT2 and its crosstalk with leptin-melanocortin signaling pathways. This tool has become pivotal in bridging genetic insights with therapeutic strategies for metabolic diseases.
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