| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIN3A |
| Uniprot No | Q96ST3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-156 aa |
| 活性数据 | MKRRLDDQESPVYAAQQRRIPGSTEAFPHQHRVLAPAPPVYEAVSETMQSATGIQYSVTPSYQVSAMPQSSGSHGPAIAAVHSSHHHPTAVQPHGGQVVQSHAHPAPPVAPVQGQQQFQRLKVVFSSFSWVKKFIFSRKYWLQLVGCGGTTSNLRY |
| 分子量 | 43.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SIN3A蛋白的3篇代表性文献及其摘要：
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1. **标题**：*"Structural analysis of the SIN3A/HDAC complex reveals a conserved repression mechanism"*
**作者**：Grimes, J.A., et al.
**摘要**：该研究通过重组表达人源SIN3A蛋白及组蛋白去乙酰化酶HDAC1.解析了二者复合物的晶体结构，揭示了SIN3A的PAH结构域如何介导与HDAC的相互作用，阐明其在染色质重塑和基因沉默中的分子机制。
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2. **标题**：*"SIN3A regulates cell cycle progression through chromatin modification in cancer cells"*
**作者**：Laurent, B.C., et al.
**摘要**：利用重组SIN3A蛋白进行体外功能实验，证明其通过招募HDAC复合物去乙酰化组蛋白H3K27.抑制细胞周期相关基因(如CDKN1A)的表达，从而促进肿瘤细胞增殖，为癌症表观遗传治疗提供靶点依据。
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3. **标题**：*"Recombinant SIN3A as a tool for mapping protein-protein interaction networks"*
**作者**：Kadamb, R., et al.
**摘要**：本研究开发了一种高效重组表达纯化SIN3A蛋白的方法，并基于质谱技术鉴定了其与多个转录因子(如FOXK1、REST)的相互作用，为解析SIN3A在不同细胞环境中的调控网络提供技术基础。
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**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science核对具体信息。研究SIN3A可重点关注其结构生物学、表观遗传调控及疾病关联方向。
SIN3A (SWI-independent 3 homolog A) is a highly conserved scaffolding protein central to transcriptional regulation in eukaryotic cells. As a core component of the SIN3/HDAC complex, it interacts with histone deacetylases (HDAC1/2) and other chromatin-modifying enzymes to repress gene expression by altering chromatin structure. This multifunctional adaptor protein contains paired amphipathic helices (PAH domains) that mediate interactions with DNA-binding transcription factors, enabling context-dependent recruitment to specific genomic loci.
Biologically, SIN3A regulates critical processes including cell cycle progression, differentiation, apoptosis, and metabolic homeostasis. It dynamically balances acetylation states of histones, linking epigenetic modifications to transcriptional outcomes. Dysregulation of SIN3A has been implicated in developmental disorders, cancers, and neurodegenerative diseases, with both tumor-suppressive and oncogenic roles reported depending on cellular context.
Recombinant human SIN3A protein is typically produced in E. coli or insect cell systems using baculovirus expression, often fused with tags (e.g., GST, His) for purification and detection. Its recombinant form enables biochemical studies of protein-protein interactions, chromatin remodeling mechanisms, and high-throughput screening for therapeutic agents targeting the SIN3/HDAC complex. Current research leverages recombinant SIN3A to dissect its role in epigenetic reprogramming and validate its potential as a therapeutic target for diseases involving transcriptional dysregulation.
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