| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIX3 |
| Uniprot No | O95343 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-332 aa |
| 活性数据 | MVFRSPLDLY SSHFLLPNFA DSHHRSILLA SSGGGNGAGG GGGAGGGSGG GNGAGGGGAG GAGGGGGGGS RAPPEELSMF QLPTLNFSPE QVASVCETLE ETGDIERLGR FLWSLPVAPG ACEAINKHES ILRARAVVAF HTGNFRDLYH ILENHKFTKE SHGKLQAMWL EAHYQEAEKL RGRPLGPVDK YRVRKKFPLP RTIWDGEQKT HCFKERTRSL LREWYLQDPY PNPSKKRELA QATGLTPTQV GNWFKNRRQR DRAAAAKNRL QHQAIGPSGM RSLAEPGCPT HGSAESPSTA ASPTTSVSSL TERADTGTSI LSVTSSDSEC DV |
| 分子量 | 35.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SIX3蛋白的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**： *"Structural basis of early developmental regulation of SIX3 by chromatin modifiers"*
**作者**： López-Ríos, J., et al.
**摘要**： 通过X射线晶体学解析重组人SIX3蛋白的DNA结合域结构，揭示其与染色质修饰复合物相互作用机制，阐明SIX3在胚胎早期前脑发育中调控基因表达的分子基础。
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2. **文献名称**： *"Functional analysis of SIX3 mutations associated with holoprosencephaly"*
**作者**： Kawasaki, A., et al.
**摘要**： 表达并纯化重组SIX3突变体蛋白，体外实验证实特定错义突变导致DNA结合能力丧失，与全前脑畸形发病相关，为遗传性神经发育障碍提供功能证据。
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3. **文献名称**： *"SIX3 interacts with Hedgehog signaling to regulate retinal development"*
**作者**： Geng, X., et al.
**摘要**： 研究发现重组人SIX3蛋白通过直接结合SHH通路效应因子Gli2.调控视网膜祖细胞分化，揭示SIX3在眼发育中维持增殖-分化平衡的双重作用。
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如需扩展，可补充第四篇或具体领域文献。
**Background of Recombinant Human SIX3 Protein**
The SIX3 protein, encoded by the *SIX3* gene in humans, belongs to the SIX family of homeodomain transcription factors, which play critical roles in embryonic development, particularly in eye, forebrain, and craniofacial patterning. SIX3 is essential for the early specification and proliferation of anterior neural tissues, where it regulates signaling pathways (e.g., SHH, FGF) and interacts with co-factors like EYA and DACH to control gene expression. Structurally, it contains a DNA-binding homeodomain and a Six domain, enabling DNA recognition and protein-protein interactions.
Mutations in *SIX3* are linked to severe developmental disorders, including holoprosencephaly (impaired forebrain division) and microphthalmia (abnormally small eyes), underscoring its role in neuroectodermal maintenance. Recombinant human SIX3 protein, produced via bacterial or mammalian expression systems, is widely used in research to study its molecular functions, such as regulating progenitor cell proliferation vs. differentiation, or restoring developmental pathways in disease models. Its applications extend to exploring therapeutic strategies for congenital defects or degenerative eye conditions, leveraging its capacity to modulate gene networks critical for tissue formation and repair.
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