| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIX6 |
| Uniprot No | O95475 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-246 aa |
| 活性数据 | MFQLPILNFS PQQVAGVCET LEESGDVERL GRFLWSLPVA PAACEALNKN ESVLRARAIV AFHGGNYREL YHILENHKFT KESHAKLQAL WLEAHYQEAE KLRGRPLGPV DKYRVRKKFP LPRTIWDGEQ KTHCFKERTR HLLREWYLQD PYPNPSKKRE LAQATGLTPT QVGNWFKNRR QRDRAAAAKN RLQQQVLSQG SGRALRAEGD GTPEVLGVAT SPAASLSSKA ATSAISITSS DSECDI |
| 分子量 | 27.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SIX6蛋白的模拟参考文献及摘要概括：
1. **"Functional analysis of recombinant human SIX6 in ocular development"**
- **作者**: Smith A, et al.
- **摘要**: 研究重组人SIX6蛋白在眼部发育中的调控作用，揭示其通过与靶基因相互作用调控晶状体和视神经发育，并发现突变体SIX6与先天性青光眼的关联。
2. **"Expression and purification of bioactive recombinant human SIX6 in E. coli"**
- **作者**: Lee C, et al.
- **摘要**: 开发了利用大肠杆菌表达系统高效制备重组人SIX6蛋白的方法，通过体外活性实验验证该蛋白对细胞增殖的抑制作用，为其临床应用提供技术基础。
3. **"SIX6 as a novel oncoprotein: Role in glioma progression"**
- **作者**: Zhang Y, et al.
- **摘要**: 发现重组人SIX6蛋白在胶质瘤细胞中异常高表达，通过激活EGFR通路促进肿瘤侵袭，提出SIX6作为潜在治疗靶点。
4. **"SIX6 genetic variants and protein dysregulation in type 2 diabetes"**
- **作者**: Patel R, et al.
- **摘要**: 通过人群队列分析，揭示SIX6基因突变导致蛋白功能缺陷与胰岛素抵抗的关联，动物模型证实重组SIX6可改善代谢异常。
注：以上为基于知识推理生成的示例，实际文献需通过学术数据库检索。如需准确文献，建议使用关键词"SIX6 recombinant protein"在PubMed或Web of Science筛选近期研究。
SIX6 (Sine oculis homeobox homolog 6) is a member of the SIX gene family, encoding transcription factors characterized by a conserved DNA-binding homeodomain and a SIX domain. Primarily expressed during embryonic development, SIX6 plays a critical role in regulating eye morphogenesis, particularly in retinal precursor cell proliferation, optic nerve development, and lens formation. It interacts with EYA family proteins to modulate transcriptional activity, influencing cell differentiation pathways. In adults, SIX6 remains active in retinal ganglion cells and the pituitary gland, suggesting roles in maintaining ocular function and hormone regulation.
Mutations or dysregulation of SIX6 are linked to congenital eye disorders, such as microphthalmia, anophthalmia, and optic nerve hypoplasia. Genome-wide studies also associate SIX6 variants with primary open-angle glaucoma risk, potentially through altered retinal ganglion cell survival or intraocular pressure regulation. Recombinant human SIX6 protein, typically produced in bacterial or mammalian expression systems, serves as a tool for studying its DNA-binding properties, protein interactions, and signaling mechanisms in vitro. This recombinant form is essential for exploring therapeutic strategies, including gene therapy for developmental eye defects or neuroprotective approaches in glaucoma. Ongoing research focuses on clarifying its downstream targets and epigenetic regulatory networks in ocular diseases.
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