| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SKIIP |
| Uniprot No | Q13573 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-536 aa |
| 活性数据 | ALTSFLPAP TQLSQDQLEA EEKARSQRSR QTSLVSSRRE PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQV DSEGKIKYDA IARQGQSKDK VIYSKYTDLV PKEVMNADDP DLQRPDEEAI KEITEKTRVA LEKSVSQKVA AAMPVRAADK LAPAQYIRYT PSQQGVAFNS GAKQRVIRMV EMQKDPMEPP RFKINKKIPR GPPSPPAPVM HSPSRKMTVK EQQEWKIPPC ISNWKNAKGY TIPLDKRLAA DGRGLQTVHI NENFAKLAEA LYIADRKARE AVEMRAQVER KMAQKEKEKH EEKLREMAQK ARERRAGIKT HVEKEDGEAR ERDEIRHDRR KERQHDRNLS RAAPDKRSKL QRNENRDISE VIALGVPNPR TSNEVQYDQR LFNQSKGMDS GFAGGEDEIY NVYDQAWRGG KDMAQSIYRP SKNLDKDMYG DDLEARIKTN RFVPDKEFSG SDRRQRGREG PVQFEEDPFG LDKFLEEAKQ HGGSKRPSDS SRPKEHEHEG KKRRKE |
| 分子量 | 61.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于已知蛋白功能和重组研究推测的3篇可能相关的文献示例(请注意，文献名为模拟假设，建议通过学术数据库核实)：
1. **文献名称**: "Functional analysis of recombinant human SKIIP in mRNA splicing regulation"
**作者**: Thompson et al.
**摘要**: 通过大肠杆菌系统表达重组人SKIIP蛋白，证实其通过结合U2AF35等剪接因子调控选择性剪接，揭示了SKIIP在前体mRNA加工中的作用机制。
2. **文献名称**: "Recombinant SKIIP enhances TGF-β/Smad signaling by stabilizing Smad complexes"
**作者**: Watanabe et al.
**摘要**: 研究发现重组SKIIP在细胞模型中与Smad2/3蛋白互作，促进TGF-β信号传导，为癌症中SKIIP过表达导致的纤维化通路异常提供分子依据。
3. **文献名称**: "Crystallographic study of the SKIIP N-terminal domain expressed in mammalian cells"
**作者**: Garcia-Saez et al.
**摘要**: 利用哺乳动物细胞表达系统获得高纯度重组SKIIP蛋白，解析其N端结构域晶体结构，阐明该区域在RNA结合及蛋白质相互作用中的关键位点。
**建议**：
- 使用关键词“recombinant human SKIIP/SKIP”、“SNW1 protein”(SKIIP又名SNW1)在PubMed或Web of Science检索最新文献。
- 检查参考文献中是否涉及重组蛋白表达(如原核/真核系统)、功能(剪接调控、信号转导)或结构研究。
**Background of Recombinant Human SKIIP (SNW1) Protein**
Recombinant human SKIIP (SNW1), a synthetic form of the Ski-interacting protein, is a multifunctional nuclear protein involved in transcriptional regulation and pre-mRNA splicing. Initially identified through its interaction with the proto-oncoprotein Ski, SKIIP acts as a transcriptional coactivator or corepressor, modulating pathways like TGF-β, Wnt, and steroid hormone signaling. Structurally, it contains conserved SNW domains that mediate protein-protein and protein-RNA interactions, enabling its role in bridging transcriptional machinery and spliceosomal components.
SKIIP is critical in embryonic development, cell cycle regulation, and stress responses. Dysregulation of SKIIP has been linked to cancers, where its overexpression promotes tumor progression by enhancing oncogenic signaling or suppressing apoptosis. Recombinant SKIIP, produced using bacterial or mammalian expression systems, retains functional domains and is widely used to study its molecular mechanisms, including splicing regulation and transcriptional control.
Recent research highlights its potential as a therapeutic target, particularly in cancers dependent on aberrant splicing or TGF-β/Wnt pathways. Recombinant variants also facilitate high-throughput screening for inhibitors and structural studies to unravel its interaction networks. Overall, SKIIP remains a key focus in understanding gene expression complexity and developing precision medicine strategies.
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