| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC18A3 |
| Uniprot No | Q16572 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MESAEPAGQARAAATKLSEAVGAALQEPRRQRRLVLVIVCVALLLDNMLYMVIVPIVPDYIAHMRGGGEGPTRTPEVWEPTLPLPTPANASAYTANTSASPTAAWPAGSALRPRYPTESEDVKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLGVMFASTVLFAFAEDYATLFAARSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVALAFISFGSLVAPPFGGILYEFAGKRVPFLVLAAVSLFDALLLLAVAKPFSAAARARANLPVGTPIHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMAWLPAFVPHVLGVYLTVRLAARYPHLQWLYGALGLAVIGASSCIVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQLSLGMGLANLLYAPVLLLLRNVGLLTRSRSERDVLLDEPPQGLYDAVRLRERPVSGQDGEPRSPPGPFDECEDDYNYYYTRS |
| 分子量 | 58.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC18A3(VAChT)蛋白的3篇参考文献(标题、作者及摘要概括)：
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1. **"Functional characterization of the human vesicular acetylcholine transporter (SLC18A3) in a yeast expression system"**
*Authors: Eiden LE, Schäfer MK, Weihe E*
**摘要**: 本研究利用酵母表达系统重组表达人源SLC18A3蛋白，验证其作为囊泡乙酰胆碱转运体的功能。实验表明，重组蛋白能依赖质子梯度高效运输乙酰胆碱，为研究胆碱能系统功能障碍提供了体外模型。
2. **"Structural insights into the human VAChT transporter by cryo-EM and molecular dynamics simulations"**
*Authors: Zhang X, Wang L, Chen Q*
**摘要**: 通过冷冻电镜和分子动力学模拟解析了重组人SLC18A3蛋白的3D结构，揭示了其底物结合腔和质子偶联转运的分子机制，为靶向该蛋白的药物设计提供了结构基础。
3. **"Recombinant SLC18A3 protein expression in HEK293 cells: Role in cholinergic neurotransmission and synaptic plasticity"**
*Authors: Takamori S, Holt M, Stenius K*
**摘要**: 在HEK293细胞中成功表达功能性重组SLC18A3蛋白，证明其参与调节乙酰胆碱释放和突触可塑性，并发现其活性异常与神经退行性疾病中的胆碱能信号缺失相关。
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注：上述文献信息为示例，实际研究请通过PubMed或专业数据库查询具体论文。SLC18A3相关研究多聚焦于其在神经递质运输和疾病中的功能机制。
The solute carrier family 18 member A3 (SLC18A3), also known as the vesicular acetylcholine transporter (VAChT), is a transmembrane protein critical for acetylcholine (ACh) neurotransmission. It is primarily localized to presynaptic vesicles in cholinergic neurons, where it transports ACh from the cytoplasm into synaptic vesicles, enabling its storage and subsequent release into synaptic clefts. This process is essential for neuromuscular signaling, cognitive functions, and autonomic nervous system regulation.
SLC18A3 belongs to the SLC18 transporter family and shares structural homology with other vesicular monoamine transporters. It functions as a proton-dependent antiporter, coupling ACh uptake to the efflux of protons generated by vesicular ATPase. Dysregulation of SLC18A3 has been implicated in neurodegenerative disorders (e.g., Alzheimer’s disease), myasthenic syndromes, and autonomic dysfunction.
Recombinant human SLC18A3 protein is produced using heterologous expression systems (e.g., mammalian or insect cells) to study its biochemical properties, ligand interactions, and role in cholinergic signaling. This tool aids in drug discovery targeting ACh-related pathologies, enabling high-throughput screening of modulators and structure-function analyses. Research on recombinant SLC18A3 also contributes to understanding vesicular transport mechanisms and developing therapies for neurological and neuromuscular diseases.
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