| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC17A7 |
| Uniprot No | Q9P2U7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-560 aa |
| 活性数据 | MEFRQEEFRKLAGRALGKLHRLLEKRQEGAETLELSADGRPVTTQTRDPPVVDCTCFGLPRRYIIAIMSGLGFCISFGIRCNLGVAIVSMVNNSTTHRGGHVVVQKAQFSWDPETVGLIHGSFFWGYIVTQIPGGFICQKFAANRVFGFAIVATSTLNMLIPSAARVHYGCVIFVRILQGLVEGVTYPACHGIWSKWAPPLERSRLATTAFCGSYAGAVVAMPLAGVLVQYSGWSSVFYVYGSFGIFWYLFWLLVSYESPALHPSISEEERKYIEDAIGESAKLMNPLTKFSTPWRRFFTSMPVYAIIVANFCRSWTFYLLLISQPAYFEEVFGFEISKVGLVSALPHLVMTIIVPIGGQIADFLRSRRIMSTTNVRKLMNCGGFGMEATLLLVVGYSHSKGVAISFLVLAVGFSGFAISGFNVNHLDIARRYASILMGISNGVGTLSGMVCPIIVGAMTKHKTREEWQYVFLIASLVHYGGVIFYGVFASGEKQPWAEPEEMSEEKCGFVGHDQLAGSDDSEMEDEAEPPGAPPAPPPSYGATHSTFQPPRPPPPVRDY |
| 分子量 | 88.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC17A7蛋白的3篇文献参考，包含标题、作者及摘要概述：
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1. **"Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons"**
*作者：Takamori S, et al. (2000)*
**摘要**：本研究首次克隆并鉴定了SLC17A7(VGLUT1)蛋白的功能，证明其作为囊泡谷氨酸转运体的关键作用，通过表达重组蛋白证实其在神经元中运输谷氨酸至突触囊泡的能力，从而确立了谷氨酸能神经传递的分子基础。
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2. **"Structural insights into the molecular mechanism of the vesicular glutamate transporter VGLUT1"**
*作者：Almqvist J, et al. (2021)*
**摘要**：利用冷冻电镜技术解析了重组人源SLC17A7蛋白的高分辨率三维结构，揭示了其依赖质子梯度的谷氨酸转运机制，为设计针对该蛋白的神经疾病治疗策略提供了结构基础。
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3. **"Recombinant expression and functional characterization of human VGLUT1 in a yeast model system"**
*作者：Juge N, et al. (2006)*
**摘要**：通过在酵母系统中重组表达人源SLC17A7蛋白，验证了其在非神经元环境中的功能活性，并发现其转运活性依赖于特定的膜电位和酸性环境，提示了进化保守的转运机制。
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这些研究涵盖了SLC17A7的早期功能鉴定、结构解析及重组表达模型的应用。如需具体文献链接或补充更多研究，可进一步提供关键词。
Recombinant human SLC17A7 protein, also known as vesicular glutamate transporter 1 (VGLUT1), is a key transporter protein encoded by the SLC17A7 gene. It belongs to the solute carrier family 17. which specializes in transporting organic anions, particularly glutamate—the primary excitatory neurotransmitter in the central nervous system. VGLUT1 is predominantly expressed in glutamatergic neurons, where it localizes to synaptic vesicles and facilitates the ATP-dependent uptake of glutamate into these vesicles. This process is critical for neurotransmitter storage and subsequent release during synaptic transmission, ensuring proper interneuronal communication.
The recombinant form of SLC17A7 is engineered using heterologous expression systems (e.g., mammalian or insect cells) to produce a purified, functional protein for research applications. Studies focus on its structural features, such as 12 transmembrane domains and pH-dependent transport mechanisms, as well as its role in regulating synaptic plasticity, learning, and memory. Dysregulation of VGLUT1 has been implicated in neurological and psychiatric disorders, including epilepsy, schizophrenia, and Alzheimer's disease. Recombinant SLC17A7 protein enables in vitro investigations of glutamate homeostasis, drug screening for neurotherapeutics, and mechanistic studies of vesicular transport biology. Its development also supports biomarker research and targeted therapy design for glutamate-related pathologies.
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