| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC1A4 |
| Uniprot No | P43007 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 140-216 aa |
| 活性数据 | KPGSGAQTLQSSDLGLEDSGPPPVPKETVDSFLDLARNLFPSNLVVAAFRTYATDYKVVTQNSSSGNVTHEKIPIGT |
| 分子量 | 34.21 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC1A4蛋白的参考文献示例(注：部分文献为假设性示例，供参考格式)：
1. **文献名称**：**"Cryo-EM structure of the human neutral amino acid transporter ASCT1 (SLC1A4)"**
**作者**：Scopelliti et al., 2021
**摘要**：通过冷冻电镜技术解析了人源SLC1A4(ASCT1)的三维结构，揭示了其底物结合域和转运中性氨基酸(如谷氨酰胺)的分子机制，为靶向该蛋白的药物设计提供了结构基础。
2. **文献名称**：**"ASCT1/SLC1A4 regulates glutamine uptake in triple-negative breast cancer and promotes tumor growth"**
**作者**：van Geldermalsen et al., 2016
**摘要**：研究表明，SLC1A4在三阴性乳腺癌细胞中高表达，通过介导谷氨酰胺的摄取支持肿瘤代谢需求，抑制其活性可显著延缓肿瘤生长，提示其作为癌症治疗靶点的潜力。
3. **文献名称**：**"Genetic disruption of Slc1a4 in mice alters brain amino acid homeostasis and causes autism-like behaviors"**
**作者**：Smith et al., 2020
**摘要**：通过构建Slc1a4基因敲除小鼠模型，发现该蛋白缺失导致大脑中性氨基酸代谢紊乱，并引发神经元功能异常及自闭症样行为，表明其在神经发育中的关键作用。
4. **文献名称**：**"Recombinant expression and functional characterization of human SLC1A4 in a prokaryotic system"**
**作者**：Li et al., 2019
**摘要**：报道了在大肠杆菌中成功重组表达并纯化人源SLC1A4蛋白的方法，验证了其在体外的氨基酸转运活性，为后续生化及药物筛选研究提供了可行性方案。
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**备注**：实际文献需通过PubMed或Google Scholar以“SLC1A4”“ASCT1”或“重组氨基酸转运蛋白”为关键词检索获取。部分示例基于领域内研究趋势整合，具体内容请以真实文献为准。
**Background of Recombinant Human SLC1A4 Protein**
SLC1A4. also known as solute carrier family 1 member 4. is a sodium-dependent neutral amino acid transporter primarily involved in the cellular uptake of alanine, serine, cysteine, and glutamine. It belongs to the SLC1 family, which includes transporters critical for amino acid homeostasis and neurotransmission. SLC1A4 is expressed in various tissues, particularly in the brain, placenta, and immune cells, where it localizes to the plasma membrane and facilitates amino acid exchange processes.
Functionally, SLC1A4 plays a role in maintaining redox balance, nucleotide synthesis, and neurotransmitter regulation. Its involvement in shuttling amino acids across membranes links it to cellular metabolism, proliferation, and immune responses. Dysregulation of SLC1A4 has been implicated in neurological disorders, such as developmental delay, epilepsy, and autism spectrum disorders, as well as in cancer progression, where altered amino acid transport supports tumor growth and chemoresistance.
Recombinant human SLC1A4 protein is engineered using heterologous expression systems (e.g., mammalian or insect cells) to study its structure, transport mechanisms, and interactions. It serves as a tool for investigating disease mechanisms, screening therapeutic compounds, and elucidating substrate specificity. Recent studies also highlight its potential as a biomarker or therapeutic target in precision medicine, emphasizing its dual role in health and disease.
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