| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC25A21 |
| Uniprot No | Q9BQT8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299 aa |
| 活性数据 | MSAKPEVSLVREASRQIVAGGSAGLVEICLMHPLDVVKTRFQIQRCATDPNSYKSLVDSFRMIFQMEGLFGFYKGILPPILAETPKRAVKFFTFEQYKKLLGYVSLSPALTFAIAGLGSGLTEAIVVNPFEVVKVGLQANRNTFAEQPSTVGYARQIIKKEGWGLQGLNKGLTATLGRHGVFNMVYFGFYYNVKNMIPVNKDPILEFWRKFGIGLLSGTIASVINIPFDVAKSRIQGPQPVPGEIKYRTCFKTMATVYQEEGILALYKGLLPKIMRLGPGGAVMLLVYEYTYSWLQENW |
| 分子量 | 59.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC25A21蛋白的假设性参考文献示例(文献信息为虚构，仅供格式参考)：
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1. **文献名称**：*Functional characterization of human SLC25A21 as a mitochondrial α-ketoadipate transporter*
**作者**：Fiermonte, G., et al.
**摘要**：本研究通过重组表达人SLC25A21蛋白于大肠杆菌系统，发现其特异性地转运α-酮己二酸(α-ketoadipate)和戊二酸，揭示了其在支链氨基酸代谢中的作用，并证实其在线粒体能量代谢中的功能机制。
2. **文献名称**：*Structural insights into the SLC25A21 transporter by cryo-EM analysis*
**作者**：Reyes, N., & Kim, J.
**摘要**：利用冷冻电镜技术解析重组人SLC25A21的三维结构，发现其独特的底物结合口袋构象，为靶向该蛋白的代谢性疾病治疗提供了结构基础。
3. **文献名称**：*SLC25A21 deficiency disrupts lysine metabolism in human cells*
**作者**：Fukasawa, Y., et al.
**摘要**：通过重组蛋白表达及基因敲除实验，证明SLC25A21缺失会损害赖氨酸分解代谢，导致α-酮己二酸在线粒体内积累，提示其与特定遗传性代谢疾病的潜在关联。
4. **文献名称**：*Expression and purification optimization of recombinant SLC25A21 for drug screening*
**作者**：Palmieri, L., et al.
**摘要**：开发了重组人SLC25A21蛋白的高效真核表达系统，优化纯化流程以评估小分子化合物对其转运活性的调控作用，为开发代谢调节药物提供平台。
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**说明**：以上文献为示例性内容，实际研究需检索PubMed、Google Scholar等数据库获取真实数据。建议通过关键词“SLC25A21”、“mitochondrial carrier”、“α-ketoadipate”或“recombinant expression”筛选文献。
Recombinant human SLC25A21 protein, a member of the mitochondrial solute carrier family (SLC25), is a nuclear-encoded transporter localized in the inner mitochondrial membrane. Also known as the oxodicarboxylate carrier (ODC), it facilitates the transport of α-ketoglutarate and other dicarboxylates across the mitochondrial membrane, playing a critical role in cellular metabolism, including the tricarboxylic acid (TCA) cycle and nitrogen metabolism. Structurally, it contains six transmembrane helices forming a channel for substrate translocation, characteristic of the mitochondrial carrier family.
SLC25A21 is essential in ketone body utilization, particularly in converting α-ketoadipate into glutaryl-CoA, linking lysine and tryptophan catabolism to energy production. Dysregulation or mutations in SLC25A21 have been implicated in metabolic disorders such as glutaric aciduria type 1 (GA1), a rare inherited disease characterized by mitochondrial dysfunction and neurological complications. Recombinant forms of this protein are often produced in heterologous systems (e.g., Escherichia coli or yeast) for functional studies, enabling insights into transport mechanisms, substrate specificity, and therapeutic targeting. Its study contributes to understanding mitochondrial carrier biology and potential interventions for metabolic diseases.
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