| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC25A25 |
| Uniprot No | Q6KCM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-469 aa |
| 活性数据 | MLCLCLYVPVIGEAQTEFQYFESKGLPAELKSIFKLSVFIPSQEFSTYRQWKQKIVQAGDKDLDGQLDFEEFVHYLQDHEKKLRLVFKSLDKKNDGRIDAQEIMQSLRDLGVKISEQQAEKILKSMDKNGTMTIDWNEWRDYHLLHPVENIPEIILYWKHSTIFDVGENLTVPDEFTVEERQTGMWWRHLVAGGGAGAVSRTCTAPLDRLKVLMQVHASRSNNMGIVGGFTQMIREGGARSLWRGNGINVLKIAPESAIKFMAYEQIKRLVGSDQETLRIHERLVAGSLAGAIAQSSIYPMEVLKTRMALRKTGQYSGMLDCARRILAREGVAAFYKGYVPNMLGIIPYAGIDLAVYETLKNAWLQHYAVNSADPGVFVLLACGTMSSTCGQLASYPLALVRTRMQAQASIEGAPEVTMSSLFKHILRTEGAFGLYRGLAPNFMKVIPAVSISYVVYENLKITLGVQSR |
| 分子量 | 79.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC25A25蛋白的3篇参考文献的简要总结(注：文献为示例性虚构，实际需根据具体研究补充)：
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1. **文献名称**：*"Recombinant expression and functional characterization of human SLC25A25 as a mitochondrial calcium carrier"*
**作者**：Smith J, et al.
**摘要**：本研究通过在大肠杆菌中成功表达重组人SLC25A25蛋白，并利用脂质体包裹实验证明其具备线粒体钙离子转运活性，揭示了SLC25A25在调节细胞能量代谢中的潜在作用。
2. **文献名称**：*"SLC25A25 knockdown alters cellular metabolism and promotes apoptosis via impaired mitochondrial ATP transport"*
**作者**：Chen L, Wang H.
**摘要**：通过siRNA敲低SLC25A25表达，发现其缺失导致线粒体ATP/ADP转运效率下降，引发细胞代谢重编程和凋亡增强，提示SLC25A25在维持能量稳态中的关键性。
3. **文献名称**：*"Structural insights into the human SLC25A25 protein by cryo-EM and molecular dynamics simulations"*
**作者**：Rodriguez A, et al.
**摘要**：结合冷冻电镜和计算模型解析了重组SLC25A25蛋白的三维结构，发现其钙离子结合位点特异性，为靶向该蛋白的代谢疾病治疗提供了结构基础。
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如需具体文献，建议通过PubMed或Web of Science以关键词 **"SLC25A25" + "recombinant"/"calcium transport"/"mitochondrial carrier"** 检索近年研究。
The human SLC25A25 protein, also known as mitochondrial calcium-binding carrier 3 (SCaMC-3) or adenine nucleotide carrier 3 (ANC3), is a member of the solute carrier family 25 (SLC25). It functions as a calcium-regulated mitochondrial transporter primarily localized in the inner mitochondrial membrane. Structurally, it features six transmembrane domains and belongs to the mitochondrial carrier subfamily (MCSC) characterized by a conserved calcium-binding motif. SLC25A25 facilitates the exchange of ATP-Mg²⁺/phosphate across the mitochondrial membrane, playing a critical role in regulating energy metabolism by linking cytosolic calcium signaling to mitochondrial bioenergetics.
Studies suggest its involvement in modulating tricarboxylic acid (TCA) cycle intermediates and maintaining mitochondrial calcium homeostasis, thereby influencing cellular survival, apoptosis, and metabolic adaptation. Dysregulation of SLC25A25 has been linked to metabolic disorders, neurodegenerative diseases, and cancer progression.
Recombinant human SLC25A25 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells*) for functional studies. Purified through affinity chromatography, it serves as a tool to investigate structure-function relationships, calcium-dependent transport mechanisms, and substrate specificity. Its recombinant form has enabled *in vitro* assays, kinetic analyses, and drug screening to explore therapeutic targeting of mitochondrial transport pathways. Research on SLC25A25 continues to unveil its role as a metabolic sensor bridging calcium signaling and mitochondrial energetics in health and disease.
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