| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC2A3 |
| Uniprot No | P11169 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496 aa |
| 活性数据 | MGTQKVTPALIFAITVATIGSFQFGYNTGVINAPEKIIKEFINKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSAALPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDAGVQEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLGAYVFIIFTGFLITFLAFTFFKVPETRGRTFEDITRAFEGQAHGADRSGKDGVMEMNSIEPAKETTTNV |
| 分子量 | 80.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人SLC2A3(GLUT3)蛋白相关的文献示例及简要信息：
1. **文献名称**: "Expression and functional characterization of recombinant human GLUT3 in yeast"
**作者**: Smith et al.
**摘要**: 研究通过酵母系统成功表达重组人SLC2A3蛋白，并验证其葡萄糖转运活性，表明其在细胞能量代谢研究中的应用潜力。
2. **文献名称**: "Structural insights into human GLUT3 dynamics by cryo-EM"
**作者**: Lee & Chen
**摘要**: 利用冷冻电镜解析了SLC2A3蛋白的三维结构，揭示了其底物结合域构象变化与葡萄糖跨膜运输的分子机制。
3. **文献名称**: "Overexpression of GLUT3 in cancer cells promotes chemoresistance via enhanced glycolysis"
**作者**: Wang et al.
**摘要**: 证实肿瘤中高表达的重组SLC2A3通过增强糖酵解通路诱导化疗耐药，为靶向代谢的癌症治疗提供新思路。
4. **文献名称**: "Role of SLC2A3 in neuronal glucose uptake and Alzheimer’s disease progression"
**作者**: Kim et al.
**摘要**: 分析重组SLC2A3在神经元模型中的功能，发现其表达下调与脑葡萄糖代谢异常及阿尔茨海默病病理相关。
(注：上述文献为示例性内容，实际引用需检索PubMed或SciFinder等数据库获取真实文献。)
Recombinant human SLC2A3 protein, also known as glucose transporter type 3 (GLUT3), is a transmembrane protein encoded by the SLC2A3 gene. As a member of the solute carrier 2 (SLC2) family, GLUT3 primarily facilitates energy-independent glucose transport across cell membranes. It exhibits high affinity for glucose, ensuring efficient uptake even under low extracellular concentrations. Structurally, it comprises 12 membrane-spanning helices with intracellular N- and C-termini, a conserved motif among facilitative glucose transporters.
GLUT3 is predominantly expressed in neurons, where it plays a critical role in maintaining brain energy homeostasis by supplying glucose as the primary fuel for oxidative metabolism. Its expression is tightly regulated to meet the dynamic metabolic demands of neural activity. Dysregulation of GLUT3 has been linked to neurodegenerative disorders, such as Alzheimer’s disease, and metabolic syndromes. Additionally, some cancers overexpress GLUT3 to support their heightened glycolytic activity, a phenomenon known as the Warburg effect.
Recombinant GLUT3 is produced using heterologous expression systems (e.g., mammalian or insect cells) for functional studies, drug screening, and structural analyses. It enables researchers to investigate glucose transport mechanisms, evaluate inhibitors for therapeutic development, and model diseases associated with glucose metabolism. Purified recombinant GLUT3 often retains post-translational modifications critical for its activity, making it invaluable for in vitro and preclinical studies aiming to unravel its physiological and pathological roles.
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