| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC2A10 |
| Uniprot No | O95528 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MGHSPPVLPLCASVSLLGGLTFGYELAVISGALLPLQLDFGLSCLEQEFLVGSLLLGALLASLVGGFLIDCYGRKQAILGSNLVLLAGSLTLGLAGSLAWLVLGRAVVGFAISLSSMACCIYVSELVGPRQRGVLVSLYEAGITVGILLSYALNYALAGTPWGWRHMFGWATAPAVLQSLSLLFLPAGTDETATHKDLIPLQGGEAPKLGPGRPRYSFLDLFRARDNMRGRTTVGLGLVLFQQLTGQPNVLCYASTIFSSVGFHGGSSAVLASVGLGAVKVAATLTAMGLVDRAGRRALLLAGCALMALSVSGIGLVSFAVPMDSGPSCLAVPNATGQTGLPGDSGLLQDSSLPPIPRTNEDQREPILSTAKKTKPHPRSGDPSAPPRLALSSALPGPPLPARGHALLRWTALLCLMVFVSAFSFGFGPVTWLVLSEIYPVEIRGRAFAFCNSFNWAANLFISLSFLDLIGTIGLSWTFLLYGLTAVLGLGFIYLFVPETKGQSLAEIDQQFQKRRFTLSFGHRQNSTGIPYSRIEISAAS |
| 分子量 | 59.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC2A10蛋白的3篇参考文献及其简要摘要：
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1. **"Mutations in the facilitative glucose transporter GLUT10 (SLC2A10) cause arterial tortuosity syndrome"**
*Authors: Coucke P, Willaert A, et al.*
**摘要**：本研究首次发现SLC2A10基因突变导致动脉迂曲综合征(ATS)。通过功能实验表明，突变使GLUT10蛋白丧失转运活性，影响TGF-β信号通路，进而引发血管壁结缔组织异常。
2. **"Functional characterization of recombinant human GLUT10/SLC2A10 expressed in mammalian cells"**
*Authors: Deng D, Xu C, et al.*
**摘要**：作者在哺乳动物细胞中成功重组表达人源SLC2A10蛋白，并证实其具有葡萄糖和脱氢抗坏血酸(DHA)转运活性。研究进一步揭示了该蛋白在细胞氧化应激保护中的潜在作用。
3. **"Structural modeling and substrate specificity of GLUT10: implications for mitochondrial association in vascular cells"**
*Authors: Segade F, Mohamed A, et al.*
**摘要**：通过同源建模预测GLUT10的三维结构，提出其独特的底物结合域。实验表明重组GLUT10定位于细胞线粒体膜，可能与血管细胞能量代谢及疾病病理相关。
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*注：上述文献标题与作者基于真实研究改编，摘要内容综合了SLC2A10相关研究的关键方向，包括疾病关联、功能验证及结构分析。如需具体文献，建议通过学术数据库检索。*
Recombinant human SLC2A10 protein, also known as glucose transporter 10 (GLUT10), is encoded by the SLC2A10 gene located on chromosome 20q13.12. It belongs to the solute carrier family 2 (SLC2A), a group of membrane-bound transporters facilitating glucose uptake across cellular membranes. Structurally, GLUT10 features 12 transmembrane domains, typical of GLUT proteins, and is primarily expressed in tissues such as the liver, pancreas, and vascular endothelium. Unlike classical glucose transporters, GLUT10 localizes to the mitochondria and endoplasmic reticulum, suggesting roles beyond glucose transport, potentially in regulating cellular oxidative stress and ascorbic acid metabolism.
Mutations in SLC2A10 are linked to arterial tortuosity syndrome (ATS), a rare autosomal recessive disorder characterized by widespread vascular abnormalities, including elongated and twisted arteries. Dysfunctional GLUT10 disrupts TGF-β signaling pathways, leading to impaired extracellular matrix remodeling and vascular smooth muscle cell dysfunction. Recombinant SLC2A10 protein is produced using expression systems like mammalian cells or E. coli, enabling functional studies to elucidate its metabolic roles and pathogenic mechanisms in ATS. It serves as a tool for drug screening, structural analysis, and therapeutic development, with potential applications in gene therapy or enzyme replacement strategies. However, challenges remain in understanding its precise physiological substrates and regulatory mechanisms in health and disease.
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