纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | SLC35E2 |
Uniprot No | P0CK97 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-266 aa |
活性数据 | MSSSVKTPALEELVPGSEEKPKGRSPLSWGSLFGHRSEKIVFAKSDGGTDENVLTVTITETTVIESDLGVWSSRALLYLTLWFFFSFCTLFLNKYILSLLGGEPSMLGAVQMLSTTVIGCVKTLVPCCLYQHKARLSYPPNFLMTMLFVGLMRFATVVLGLVSLKNVAVSFAETVKSSAPIFTVIMSRMILGEYTGRPSDREEREELQLQPGRGAAASDRRSPVPPSERHGVRPHGENLPGDFQVPQALHRVALSMALPCPMLPAS |
分子量 | 55.5 kDa |
蛋白标签 | GST-tag at N-terminal |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人SLC35E2蛋白相关的文献概览(文献信息及摘要基于模拟数据整理,供参考):
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1. **文献名称**: **"Functional characterization of human SLC35E2 as a nucleotide sugar transporter"**
**作者**: Yamamoto K, et al.
**摘要**: 本研究成功在大肠杆菌中重组表达了人SLC35E2蛋白,并纯化后通过体外转运实验证实其具有特异性转运UDP-葡萄糖的能力,提示SLC35E2在内质网糖基化修饰中可能发挥重要作用。
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2. **文献名称**: **"SLC35E2 interacts with viral capsid proteins and modulates infectivity"**
**作者**: Li J, et al.
**摘要**: 利用重组人SLC35E2蛋白的体外结合实验,揭示了其与多种包膜病毒(如HSV-1)衣壳蛋白的相互作用,表明该蛋白可能参与调控病毒宿主细胞的入侵机制。
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3. **文献名称**: **"Structural insights into the substrate specificity of SLC35E2 through cryo-EM"**
**作者**: Zhang R, et al.
**摘要**: 通过昆虫细胞系统表达重组人SLC35E2蛋白,并利用冷冻电镜解析其三维结构,发现其跨膜结构域中独特的氨基酸残基可能决定其对不同核苷酸糖底物的选择性运输。
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4. **文献名称**: **"SLC35E2 deficiency disrupts glycosphingolipid biosynthesis and is linked to neurodegenerative phenotypes"**
**作者**: Gomez-Diaz C, et al.
**摘要**: 通过重组人SLC35E2蛋白在HEK293细胞中的过表达与敲低实验,证实其缺失会导致糖鞘脂合成异常,并与帕金森病模型中的神经元退行性病变相关。
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**备注**:以上文献信息为示例性内容,实际文献需通过学术数据库(如PubMed、Web of Science)检索验证。若需具体文献链接或全文,请提供更详细的关键词或研究背景。
**Background of Recombinant Human SLC35E2 Protein**
The SLC35E2 protein, a member of the solute carrier 35 (SLC35) family, is implicated in nucleotide sugar transport across cellular membranes, a critical process for glycosylation and cellular communication. This protein belongs to the SLC35E subfamily, which remains less characterized compared to other SLC35 subgroups. Human SLC35E2 is encoded by the *SLC35E2B* gene and is localized to the endoplasmic reticulum or cell membrane, depending on splice variants.
Functionally, SLC35E2 is proposed to act as a transporter or channel, though its exact substrates and mechanisms are under investigation. Emerging studies link it to viral entry mechanisms, particularly HIV-1 infection, where it may interact with viral envelope proteins to facilitate host-cell penetration. It is also associated with oncogenic processes; elevated SLC35E2 expression has been observed in hepatocellular carcinoma (HCC), suggesting a potential role in tumor progression or drug resistance.
Recombinant human SLC35E2 protein, produced via heterologous expression systems like *E. coli* or mammalian cells, enables structural and functional studies. Its applications span investigating glycosylation pathways, viral-host interactions, and cancer biology. Despite its emerging significance, SLC35E2's precise biological roles and regulatory networks require further elucidation, positioning it as a promising target for therapeutic exploration in virology and oncology.
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