| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC39A10 |
| Uniprot No | Q9ULF5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-831 aa |
| 活性数据 | MKVHMHTKFCLICLLTFIFHHCNHCHEEHDHGPEALHRQHRGMTELEPSKFSKQAAENEKKYYIEKLFERYGENGRLSFFGLEKLLTNLGLGERKVVEINHEDLGHDHVSHLDILAVQEGKHFHSHNHQHSHNHLNSENQTVTSVSTKRNHKCDPEKETVEVSVKSDDKHMHDHNHRLRHHHRLHHHLDHNNTHHFHNDSITPSERGEPSNEPSTETNKTQEQSDVKLPKGKRKKKGRKSNENSEVITPGFPPNHDQGEQYEHNRVHKPDRVHNPGHSHVHLPERNGHDPGRGHQDLDPDNEGELRHTRKREAPHVKNNAIISLRKDLNEDDHHHECLNVTQLLKYYGHGANSPISTDLFTYLCPALLYQIDSRLCIEHFDKLLVEDINKDKNLVPEDEANIGASAWICGIISITVISLLSLLGVILVPIINQGCFKFLLTFLVALAVGTMSGDALLHLLPHSQGGHDHSHQHAHGHGHSHGHESNKFLEEYDAVLKGLVALGGIYLLFIIEHCIRMFKHYKQQRGKQKWFMKQNTEESTIGRKLSDHKLNNTPDSDWLQLKPLAGTDDSVVSEDRLNETELTDLEGQQESPPKNYLCIEEEKIIDHSHSDGLHTIHEHDLHAAAHNHHGENKTVLRKHNHQWHHKHSHHSHGPCHSGSDLKETGIANIAWMVIMGDGIHNFSDGLAIGAAFSAGLTGGISTSIAVFCHELPHELGDFAVLLKAGMTVKQAIVYNLLSAMMAYIGMLIGTAVGQYANNITLWIFAVTAGMFLYVALVDMLPEMLHGDGDNEEHGFCPVGQFILQNLGLLFGFAIMLVIALYEDKIVFDIQF |
| 分子量 | 120.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC39A10蛋白的3篇参考文献的简要列举：
1. **文献名称**：*SLC39A10通过调节锌离子稳态促进肿瘤生长和转移*
**作者**：Kim H, et al.
**摘要**：研究发现，SLC39A10通过维持细胞内锌离子平衡，激活MAPK/ERK信号通路，从而促进结肠癌细胞的增殖和转移;通过基因敲除实验证实其可作为潜在治疗靶点。
2. **文献名称**：*SLC39A10在肥胖相关代谢紊乱中的功能研究*
**作者**：Wang Y, et al.
**摘要**：通过构建脂肪细胞特异性敲除小鼠模型，发现SLC39A10缺失会加重胰岛素抵抗和脂肪组织炎症，表明其在脂代谢和肥胖相关疾病中起保护作用。
3. **文献名称**：*SLC39A10的晶体结构解析及其锌转运机制*
**作者**：Smith J, et al.
**摘要**：首次报道人SLC39A10蛋白的晶体结构，揭示其跨膜锌离子通道的构象变化，为靶向该蛋白的药物设计提供结构基础。
4. **文献名称**：*SLC39A10调控巨噬细胞极化及炎症反应*
**作者**：Chen L, et al.
**摘要**：实验表明SLC39A10通过锌离子依赖的方式调节巨噬细胞向M1型极化，加重脓毒症模型中的全身炎症反应，提示其参与免疫调控。
(注：以上文献为示例性内容，实际引用需核实真实存在的出版物。)
**Background of Recombinant Human SLC39A10 Protein**
SLC39A10. a member of the solute carrier family 39 (SLC39), is a zinc transporter protein involved in cellular zinc homeostasis. It facilitates the uptake of zinc across cell membranes, playing a critical role in maintaining intracellular zinc levels essential for numerous biological processes, including enzyme function, signal transduction, and gene regulation. Structurally, SLC39A10 contains multiple transmembrane domains and interacts with zinc ions via conserved metal-binding residues.
Dysregulation of SLC39A10 has been linked to various pathological conditions. Studies suggest its overexpression in certain cancers, such as breast and liver cancer, where it promotes tumor progression by enhancing zinc-dependent signaling pathways like NF-κB and MAPK. Conversely, reduced SLC39A10 activity is associated with neurodegenerative disorders, possibly due to impaired zinc buffering in neuronal cells.
Recombinant human SLC39A10 protein is produced using heterologous expression systems (e.g., Escherichia coli or mammalian cell lines) to enable functional and structural studies. Its applications include elucidating zinc transport mechanisms, screening therapeutic agents targeting zinc-related diseases, and exploring its role in metabolic or immune regulation. Researchers also utilize purified SLC39A10 to investigate its interactions with metal ions, membrane proteins, and regulatory partners, advancing insights into metal biology and disease pathogenesis.
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