| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC6A16 |
| Uniprot No | Q9GZN6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-659 aa |
| 活性数据 | MKTEAQPSTSLLANTSWTGTVISDSVPGSQTWEDKGSLTRSATSWTSEAQVSAARVAEAQARTSQPKQISVLEALTASALNQKPTHEKVQMTEKKESEVLLARPFWSSKTEYILAQVGFSMKPSCLWRFAYLWLNSGGCSFAAIYIFMLFLVGVPLLFLEMAAGQSMRQGGMGVWKIIAPWIGGVGYSSFMVCFILGLYFNVVNSWIIFYMSQSFQFPVPWEKCPLTMNSSGFDPECERTTPSIYFWYQQALKASDRIEDGGSPVYSLVLPFFLCWCLVGAFMINGLKSTGKVIYVLVLLPCFIIVGFFIRTLLLEGAKFGLQQLVVAKISDVYNMSVWSLAGGQVLSNTGIGLGSVASLASYMPQSNNCLSDAFLVSVINLLTLLVFTSFNFCVLGFWATVITHRCCERNAEILLKLINLGKLPPDAKPPVNLLYNPTSIYNAWLSGLPQHIKSMVLREVTECNIETQFLKASEGPKFAFLSFVEAMSFLPPSVFWSFIFFLMLLAMGLSSAIGIMQGIITPLQDTFSFFRKHTKLLIVGVFLLMFVCGLFFTRPSGSYFIRLLSDYWIVFPIIVVVVFETMAVSWAYGARRFLADLTILLGHPISPIFGWLWPHLCPVVLLIIFVTMMVHLCMKPITYMSWDSSTVSLPQDPEPLWE |
| 分子量 | 99.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC6A16蛋白的3篇文献摘要示例(基于领域知识模拟，可能非真实文献)：
1. **"Functional characterization of recombinant human SLC6A16 as a neutral amino acid transporter"**
*作者：Lee et al. (2020)*
摘要：通过重组表达SLC6A16蛋白于HEK293细胞，证明其介导中性氨基酸(如脯氨酸、甘氨酸)的钠离子依赖性转运，并揭示其在中枢神经系统的潜在生理作用。
2. **"Structural insights into SLC6A16 via cryo-EM and its role in tumor metabolism"**
*作者：Zhang et al. (2021)*
摘要：利用冷冻电镜解析重组SLC6A16的分子结构，发现其具有独特的底物结合域，并在肿瘤细胞系中验证其通过调控氨基酸代谢促进细胞增殖的功能。
3. **"SLC6A16 recombinant protein expression and interaction with viral glycoproteins"**
*作者：Kumar et al. (2019)*
摘要：在大肠杆菌中成功表达重组SLC6A16胞外段，证实其与多种病毒包膜蛋白特异性结合，暗示其可能作为病毒感染的宿主受体。
注：以上文献为模拟生成，如需真实文献建议通过PubMed或Google Scholar搜索关键词 **SLC6A16 recombinant protein expression/function** 获取。
**Background of Recombinant Human SLC6A16 Protein**
SLC6A16. a member of the solute carrier family 6 (SLC6), is a sodium- and chloride-dependent transporter predominantly expressed in tissues like the testis, thyroid, and certain cancer cells. It shares structural and functional homology with other SLC6 transporters, which mediate the uptake of neurotransmitters, amino acids, and osmolytes. As a recombinant protein, SLC6A16 is produced in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to enable detailed study of its biochemical properties and physiological roles.
Structurally, SLC6A16 features 12 transmembrane domains with intracellular N- and C-termini, and conserved glycosylation sites on its extracellular loops. Its substrate specificity remains unclear, though it may transport small molecules like amino acids or carnitine. Notably, SLC6A16 is upregulated in cancers (e.g., gliomas, lung adenocarcinoma), suggesting a potential role in tumor metabolism or progression. However, its precise biological functions in normal and pathological states are poorly understood.
Recombinant SLC6A16 protein facilitates research on ligand interactions, transport mechanisms, and drug discovery. It also aids in generating antibodies and exploring its therapeutic or diagnostic relevance in cancer. Despite progress, further studies are needed to fully elucidate its substrates, regulation, and pathophysiological significance.
×
