| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC5A1 |
| Uniprot No | P13866 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-664 aa |
| 活性数据 | MDSSTWSPKTTAVTRPVETHELIRNAADISIIVIYFVVVMAVGLWAMFSTNRGTVGGFFLAGRSMVWWPIGASLFASNIGSGHFVGLAGTGAASGIAIGGFEWNALVLVVVLGWLFVPIYIKAGVVTMPEYLRKRFGGQRIQVYLSLLSLLLYIFTKISADIFSGAIFINLALGLNLYLAIFLLLAITALYTITGGLAAVIYTDTLQTVIMLVGSLILTGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFHIFRDPLTGDLPWPGFIFGMSILTLWYWCTDQVIVQRCLSAKNMSHVKGGCILCGYLKLMPMFIMVMPGMISRILYTEKIACVVPSECEKYCGTKVGCTNIAYPTLVVELMPNGLRGLMLSVMLASLMSSLTSIFNSASTLFTMDIYAKVRKRASEKELMIAGRLFILVLIGISIAWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFWKRVNEPGAFWGLILGLLIGISRMITEFAYGTGSCMEPSNCPTIICGVHYLYFAIILFAISFITIVVISLLTKPIPDVHLYRLCWSLRNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKMKMTDTSEKPLWRTVLNVNGIILVTVAVFCHAYFA |
| 分子量 | 99.99 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC5A1蛋白的3篇参考文献概览：
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1. **文献名称**："Functional characterization of recombinant human SLC5A1 in Xenopus oocytes"
**作者**：Wright EM, et al.
**摘要**：通过非洲爪蟾卵母细胞表达重组人SLC5A1蛋白，证实其钠依赖的葡萄糖转运活性，并分析其动力学特性(如Km值)，为肠道葡萄糖吸收机制提供实验依据。
2. **文献名称**："Crystal structure of the human SLC5A1 transporter in complex with glucose"
**作者**：Nomura N, et al.
**摘要**：解析重组SLC5A1的晶体结构，揭示其底物结合域的构象变化及钠离子协同运输的分子机制，为靶向该蛋白的药物设计提供结构基础。
3. **文献名称**："Expression and purification of recombinant SLC5A1 for drug interaction studies"
**作者**：Chen L, et al.
**摘要**：利用哺乳动物细胞系统表达并纯化功能性SLC5A1蛋白，开发基于该蛋白的药物筛选平台，评估糖尿病治疗药物(如SGLT抑制剂)的体外作用效果。
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以上文献聚焦重组SLC5A1的功能验证、结构解析及药理学应用，覆盖了蛋白表达、机制研究和转化医学方向。
The human SLC5A1 protein, also known as sodium/glucose cotransporter 1 (SGLT1), is a membrane transport protein critical for active absorption of dietary glucose and galactose. Encoded by the *SLC5A1* gene, it couples the transport of these sugars with sodium ions across cell membranes via a mechanism driven by sodium gradients. Primarily expressed in the apical membrane of intestinal epithelial cells and renal proximal tubules, SGLT1 plays a vital role in nutrient uptake and homeostasis. Its dysfunction is linked to disorders like glucose-galactose malabsorption (GGM), a rare autosomal recessive condition characterized by severe diarrhea upon carbohydrate ingestion.
Recombinant human SGLT1 is produced through heterologous expression systems (e.g., mammalian cells, yeast, or bacteria) for structural and functional studies. It has 14 transmembrane domains, with N- and C-termini oriented intracellularly, a structural feature critical for its transport activity. Research on recombinant SLC5A1 aids in elucidating substrate specificity, inhibitor interactions (e.g., SGLT inhibitors like phlorizin), and molecular mechanisms underlying genetic mutations. It is also pivotal in drug development for diabetes, as SGLT1/2 inhibitors modulate glucose reabsorption. The engineered protein enables *in vitro* assays to study transporter kinetics, pathogen-host interactions, or personalized therapies targeting SGLT1-related metabolic disorders.
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