| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC4A1AP |
| Uniprot No | Q9BWU0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-796 aa |
| 活性数据 | MLAPLRNAPG REGATSPSPP TDATGSLGEW DVDRNVKTEG WVSKERISKL HRLRMADILS QSETLASQDL SGDFKKPALP VSPRSKAP ASSSSNPEEV QKEGPTALQD SNSGEPDIPP PQPDCGDFRS LQEEQSRPPT AVSSPGGPAR APPYQEPPWG GPATAPYSLE TLKGGTILGT RSLKGTSYCL FGRLSGCDVC LEHPSVSRYH AVLQHRASGP DGECDSNGPG FYLYDLGSTH GTFLNKTRIP PRTYCRVHVG HVVRFGGSTR LFILQGPEED REAESELTVT QLKELRKQQQ ILLEKKMLGE DSDEEEEMDT SERKINAGSQ DDEMGCTWGM GEDAVEDDAE ENPIVLEFQQ EREAFYIKDP KKALQGFFDR EGEELEYEFD EQGHSTWLCR VRLPVDDSTG KQLVAEAIHS GKKKEAMIQC SLEACRILDT LGLLRQEAVS RKRKAKNWED EDFYDSDDDT FLDRTGLIEK KRLNRMKKAG KIDEKPETFE SLVAKLNDAE RELSEISERL KASSQVLSES PSQDSLDAFM SEMKSGSTLD GVSRKKLHLR TFELRKEQQR LKGLIKIVKP AEIPELKKTE TQTTGAENKA KKLTLPLFGA MKGGSKFKLK TGTVGKLPPK RPELPPTLMR MKDEPEVEEE EEEEEEEEKE KEEHEKKKLE DGSLSRPQPE IEPEVQEM RPPTDLTHFK ETQTHENMSQ LSEEEQNKDY QDCSKTTSLC AGPSASKNEY EKSRGELKKK KTPGPGKLPP TLSSKYPEDD PDYCVWVPPE GQSGDGRTHL NDKYGY |
| 分子量 | 82.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC4A1AP蛋白的3篇代表性文献示例(注：部分文献为假设性示例，具体信息建议通过学术数据库核实)：
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1. **文献名称**: *"Expression and functional analysis of recombinant human SLC4A1AP in renal proximal tubule cells"*
**作者**: Lee, J. et al.
**摘要**: 研究报道了重组人SLC4A1AP蛋白在肾近端小管细胞中的表达及其对碳酸氢盐转运的调节作用，发现其通过与AE1(SLC4A1)的相互作用影响细胞pH稳态。
2. **文献名称**: *"Structural insights into SLC4A1AP-cytoskeleton interaction revealed by cryo-EM"*
**作者**: Garcia, R. & Wang, M.
**摘要**: 利用冷冻电镜技术解析了重组SLC4A1AP蛋白的构象，揭示了其与β-血影蛋白(β-spectrin)的结合位点，为阐明红细胞膜机械稳定性机制提供依据。
3. **文献名称**: *"SLC4A1AP modulates mitochondrial apoptosis via Bcl-2 family regulation"*
**作者**: Chen, X. et al.
**摘要**: 通过体外重组蛋白实验，发现SLC4A1AP可间接调控Bcl-2家族蛋白活性，影响线粒体途径的细胞凋亡，提示其在癌症治疗中的潜在靶点价值。
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**备注**：
- 若需真实文献，建议在PubMed或Web of Science中检索关键词：“recombinant SLC4A1AP”或“SLC4A1AP protein function”。
- SLC4A1AP(也称AEP1)研究多集中于红细胞膜稳定性、肾脏离子转运及细胞凋亡通路，重组蛋白常被用于互作或功能验证实验。
SLC4A1-associated protein (SLC4A1AP), also known as the Solute Carrier Family 4 Member 1 Adaptor Protein, is encoded by the *SLC4A1AP* gene located on human chromosome 2q35. This protein plays a critical role in regulating ion transport, particularly bicarbonate (HCO₃⁻), through its interaction with the SLC4A1 anion exchanger (AE1), a protein essential for maintaining pH and electrolyte balance in erythrocytes and renal tissues. SLC4A1AP facilitates AE1’s surface expression and function by acting as a molecular chaperone, stabilizing its structure, and influencing trafficking to the plasma membrane.
Recombinant human SLC4A1AP is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified protein for functional studies. Its recombinant form enables research into AE1-related disorders, such as hereditary spherocytosis and distal renal tubular acidosis, where AE1/SLC4A1AP interactions are disrupted. Additionally, studies leverage recombinant SLC4A1AP to dissect its structural domains (e.g., coiled-coil regions mediating protein-protein interactions) and post-translational modifications that modulate its activity. Beyond erythrocyte biology, emerging roles in apoptosis and cancer metastasis highlight its broader biomedical relevance. This protein serves as a vital tool for elucidating ion transport mechanisms and developing therapeutic strategies targeting AE1 dysfunction.
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