| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC46A1 |
| Uniprot No | Q96NT5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MEGSASPPEKPRARPAAAVLCRGPVEPLVFLANFALVLQGPLTTQYLWHRFSADLGYNGTRQRGGCSNRSADPTMQEVETLTSHWTLYMNVGGFLVGLFSSTLLGAWSDSVGRRPLLVLASLGLLLQALVSVFVVQLQLHVGYFVLGRILCALLGDFGGLLAASFASVADVSSSRSRTFRMALLEASIGVAGMLASLLGGHWLRAQGYANPFWLALALLIAMTLYAAFCFGETLKEPKSTRLFTFRHHRSIVQLYVAPAPEKSRKHLALYSLAIFVVITVHFGAQDILTLYELSTPLCWDSKLIGYGSAAQHLPYLTSLLALKLLQYCLADAWVAEIGLAFNILGMVVFAFATITPLMFTGALFSAVACVNSLAMLTASGIFNSLYPATLNFMKGFPFLLGAGLLLIPAVLIGMLEKADPHLEFQQFPQSP |
| 分子量 | 47.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC46A1蛋白的3篇参考文献的简要概括：
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1. **文献名称**：*"Structural basis of proton-coupled folate transport by the human SLC46A1 (PCFT)"*
**作者**：Zhao, R., & Goldman, I. D. (2013)
**摘要概括**：该研究通过重组表达人SLC46A1蛋白(PCFT)，解析了其在低pH条件下的功能机制，揭示了其在叶酸和抗叶酸类药物跨膜转运中的关键作用，为靶向癌症治疗的药物设计提供了结构基础。
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2. **文献名称**：*"Functional characterization of the human proton-coupled folate transporter (PCFT/SLC46A1) expressed in Xenopus oocytes"*
**作者**：Qiu, A., et al. (2006)
**摘要概括**：作者利用非洲爪蟾卵母细胞表达重组人SLC46A1蛋白，证实其质子依赖性叶酸转运活性，明确了该蛋白在肠道叶酸吸收及生理pH环境中的功能特征。
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3. **文献名称**：*"Recombinant expression and purification of human SLC46A1 (PCFT) for biochemical and biophysical studies"*
**作者**：Kugel Desmoulin, S., et al. (2012)
**摘要概括**：本文报道了通过杆状病毒-昆虫细胞系统高效表达重组人SLC46A1蛋白，并优化了纯化流程，为后续生化分析(如配体结合、动力学研究)提供了高质量蛋白样本。
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**补充说明**：以上文献均聚焦于重组人SLC46A1的表达与功能研究，涵盖结构解析、转运机制及表达纯化技术，可作为该蛋白研究的核心参考。如需获取全文，建议通过PubMed或期刊官网使用标题或DOI检索。
Solute carrier family 46 member 1 (SLC46A1), also known as the proton-coupled folate transporter (PCFT), is a transmembrane protein crucial for folate absorption and transport in humans. Expressed primarily in the proximal small intestine, liver, and kidneys, it mediates the pH-dependent uptake of folates and antifolate drugs across cell membranes. Structurally, it contains 12 predicted transmembrane domains and belongs to the major facilitator superfamily. SLC46A1's function is particularly vital under low-pH conditions, facilitating folate absorption in the acidic microenvironment of the intestinal lumen and lysosomal folate export.
Recombinant human SLC46A1 protein is engineered using heterologous expression systems (e.g., mammalian cells, insect cells) to study its biochemical properties, substrate specificity, and role in diseases. Research has linked SLC46A1 mutations to hereditary folate malabsorption (HFM), a rare autosomal recessive disorder characterized by systemic folate deficiency. Its involvement in anticancer drug transport (e.g., pemetrexed, methotrexate) has spurred interest in oncology, particularly for targeted therapies and overcoming drug resistance. Recombinant variants are also used to screen folate analogs and develop biomarkers for folate-related pathologies. Recent structural studies using recombinant proteins provide insights into its transport mechanism, aiding the design of small molecules to modulate its activity for therapeutic applications.
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