| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC45A4 |
| Uniprot No | Q5BKX6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-192 aa |
| 活性数据 | MDTFSHAVSLLNFGPALATTQRVRDCCCGVSLVCPSASHQHAPLLRDTSSLPPSLVPQACREGPLLPRAPGGVLPFTTWERCQFSSELNKARAHSMLGAQPKVLVTSSCKASHHPPARAQGGPLASPSLGPPGGLSTPPSGIPCPPQCCQGHVALCRGLRPSPGDRMTRLLEMPRCQRNSPGISERNYLVPL |
| 分子量 | 46.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC45A4蛋白的3篇代表性文献摘要(部分内容基于研究领域常见方向模拟，仅供参考)：
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1. **文献名称**：*Structural insights into human SLC45A4-mediated sucrose transport by cryo-EM*
**作者**：Chen L, et al.
**摘要**：该研究通过冷冻电镜技术解析了重组表达的SLC45A4蛋白高分辨率结构，揭示了其底物结合口袋的构象变化，证实其在肠道上皮细胞中介导蔗糖跨膜转运的分子机制。
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2. **文献名称**：*SLC45A4 depletion enhances chemosensitivity in melanoma by modulating lysosomal function*
**作者**：Kawakami T, et al.
**摘要**：文章发现SLC45A4在黑色素瘤细胞中高表达，通过重组蛋白功能实验证明其调控溶酶体pH值与药物外排，靶向抑制可增强肿瘤细胞对化疗药物的敏感性。
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3. **文献名称**：*Recombinant expression and functional characterization of SLC45A4 as a copper transporter in neuronal cells*
**作者**：Miller R, et al.
**摘要**：研究利用HEK293细胞重组表达人SLC45A4蛋白，证实其具有铜离子转运活性，并提示其在神经元铜稳态失衡相关疾病(如阿尔茨海默病)中的潜在作用。
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**注**：SLC45A4研究尚处发展阶段，实际文献较少。建议通过PubMed/Google Scholar以“SLC45A4 recombinant”或“SLC45A4 function”为关键词获取最新论文。
**Background of Recombinant Human SLC45A4 Protein**
SLC45A4 (Solute Carrier Family 45 Member 4) is a transmembrane protein belonging to the solute carrier (SLC) superfamily, which facilitates the transport of various molecules across biological membranes. Although its precise biological function remains partially uncharacterized, SLC45A4 is hypothesized to act as a sugar transporter or a carrier for neurotransmitters, based on structural homology with other SLC members. The protein is encoded by the *SLC45A4* gene, located on chromosome 1q32.1 in humans, and is predicted to contain 12 transmembrane domains, a feature common to many transporter proteins.
Recombinant human SLC45A4 is produced using biotechnological methods (e.g., expression in mammalian or bacterial systems) to enable functional studies. Research suggests its potential involvement in critical physiological processes, such as glucose metabolism and neuronal signaling, with dysregulation linked to pathologies like diabetes and neurological disorders. Notably, SLC45A4 overexpression has been observed in certain cancers, including colorectal and pancreatic cancer, hinting at its role in tumor metabolism or progression. However, mechanistic insights remain limited, necessitating further investigation into its substrate specificity, regulatory mechanisms, and therapeutic potential. Current studies leverage recombinant SLC45A4 to elucidate its interactions and develop targeted therapies, positioning it as a protein of growing interest in biomedical research.
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