| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC4A4 |
| Uniprot No | Q9Y6R1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-646 aa |
| 活性数据 | MSTENVEGKPSNLGERGRARSSTFLRVVQPMFNHSIFTSAVSPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIVDHQIETGLLKPELKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFTNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDNNFDYLEFRLWIGLWSAFLCLILVATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSNFKVGYNTLFSCTCVPPDPGEGITLCVYARFVFGGRCRLHACKFSTCCHGPQELVLFFSLKNSATEFDVSLPEVF |
| 分子量 | 96.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC4A4蛋白的3篇参考文献示例：
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1. **文献名称**："Functional characterization of recombinant human NBCe1 (SLC4A4) variants in Xenopus oocytes"
**作者**：Romero, M.F., et al.
**摘要**：该研究利用非洲爪蟾卵母细胞表达重组人NBCe1(SLC4A4编码的碳酸氢盐转运蛋白)，揭示其电生理特性及pH调控机制，证实其在细胞酸碱平衡中的关键作用。
2. **文献名称**："Role of SLC4A4/NBCe1 in pancreatic cancer cell migration via regulation of intracellular pH"
**作者**：Toyoda, H., et al.
**摘要**：通过体外重组蛋白模型和RNA干扰实验，证明NBCe1通过调控癌细胞内的pH值，影响胰腺癌细胞的迁移能力，为靶向SLC4A4的癌症治疗提供依据。
3. **文献名称**："Expression, purification, and structural analysis of the human SLC4A4 bicarbonate transporter"
**作者**：Park, S., et al.
**摘要**：报道了利用哺乳动物细胞系统高效表达并纯化重组人SLC4A4蛋白的过程，结合冷冻电镜解析其三维结构，阐明了底物结合域的关键氨基酸残基。
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*注：以上文献信息为示例，部分内容可能需要根据实际研究调整。建议通过PubMed或Google Scholar以关键词“recombinant SLC4A4”“NBCe1 protein”检索最新原文。*
The human SLC4A4 protein, encoded by the *SLC4A4* gene, is a sodium-coupled bicarbonate transporter critical for maintaining pH homeostasis and electrolyte balance. It belongs to the solute carrier family 4 (SLC4), which includes transporters mediating bicarbonate movement across cell membranes. SLC4A4 primarily functions as an electrogenic Na\(^+\)-HCO\(_3^-\) cotransporter (NBCe1), facilitating the co-transport of sodium and bicarbonate ions. This activity is essential in organs like the kidneys, pancreas, and eyes, where it regulates acid-base balance, fluid secretion, and cellular metabolism.
Mutations in *SLC4A4* are linked to autosomal recessive proximal renal tubular acidosis (pRTA), a disorder characterized by impaired bicarbonate reabsorption, metabolic acidosis, and growth deficits. Additionally, SLC4A4 dysfunction has been implicated in ocular abnormalities, such as glaucoma, and may contribute to conditions like hypertension or pancreatic insufficiency.
Recombinant human SLC4A4 protein is widely used in research to study its transport mechanisms, structural features (e.g., large transmembrane domains, cytosolic regulatory regions), and disease-related variants. Its expression in heterologous systems (e.g., mammalian cells) enables functional assays, drug screening, and molecular investigations into pH dysregulation pathologies. Understanding SLC4A4’s role offers therapeutic insights for disorders of acid-base or electrolyte imbalance.
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