关键信息
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基因名
Mcpt2
- 应用
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别名
Mcpt2；Mast cell protease 2
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种属
Mouse
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P15119
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表达区间
21-244aa
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氨基酸序列
IIGGVEAKPH SRPYMAYLKF TTKNGSKERC GGFLIAPQFV MTAAHCNGSE ISVILGAHNI NKNEPTQQII KTEKTFVHPK FQYLSGFYDI MLLKLQKKAE LNSDVDVISL PSSSDFIKPG KMCWTAGWGK TGKNNPLSVT LREVELRIMD QEACKDHSDY DYQLQVCAGS PTTSKSIGQG DSGGPLVCDS VAHGIASSYE AKAPAVFTRI SYYLPWIYKV LKSK
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分子量
26.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Mcpt2, or mast cell protease 2, is a member of the serine protease family primarily expressed in mast cells, which are critical components of the immune system. This protease plays a vital role in various physiological and pathological processes, including inflammation, allergy, and defense against pathogens. The study of Mcpt2 particularly emphasizes its involvement in modulating immune responses, especially in allergic reactions and asthma. Research has indicated that Mcpt2 can influence the activation of other immune cells, release of cytokines, and degradation of extracellular matrix components, thereby affecting tissue remodeling and inflammation. As a result, understanding the mechanisms through which Mcpt2 operates could unveil novel therapeutic targets for allergic and inflammatory diseases. Recombining Mcpt2 protein for experimental purposes offers valuable insights into its functional properties, interactions with other molecules, and potential contributions to disease pathways. This has led to increasing interest among researchers in investigating its structure, activity, and regulation, highlighting its significance in mast cell biology and the broader context of immune system regulation.
