| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC6A19 |
| Uniprot No | Q695T7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-634 aa |
| 活性数据 | MVRLVLPNPGLDARIPSLAELETIEQEEASSRPKWDNKAQYMLTCLGFCVGLGNVWRFPYLCQSHGGGAFMIPFLILLVLEGIPLLYLEFAIGQRLRRGSLGVWSSIHPALKGLGLASMLTSFMVGLYYNTIISWIMWYLFNSFQEPLPWSDCPLNENQTGYVDECARSSPVDYFWYRETLNISTSISDSGSIQWWMLLCLACAWSVLYMCTIRGIETTGKAVYITSTLPYVVLTIFLIRGLTLKGATNGIVFLFTPNVTELAQPDTWLDAGAQVFFSFSLAFGGLISFSSYNSVHNNCEKDSVIVSIINGFTSVYVAIVVYSVIGFRATQRYDDCFSTNILTLINGFDLPEGNVTQENFVDMQQRCNASDPAAYAQLVFQTCDINAFLSEAVEGTGLAFIVFTEAITKMPLSPLWSVLFFIMLFCLGLSSMFGNMEGVVVPLQDLRVIPPKWPKEVLTGLICLGTFLIGFIFTLNSGQYWLSLLDSYAGSIPLLIIAFCEMFSVVYVYGVDRFNKDIEFMIGHKPNIFWQVTWRVVSPLLMLIIFLFFFVVEVSQELTYSIWDPGYEEFPKSQKISYPNWVYVVVVIVAGVPSLTIPGYAIYKLIRNHCQKPGDHQGLVSTLSTASMNGDLKY |
| 分子量 | 97.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人SLC6A19蛋白相关的文献概览，涵盖功能研究和结构分析方向：
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1. **文献名称**：*"SLC6A19 is a neutral amino acid transporter in the small intestine and kidney"*
**作者**：Bröer, S. et al.
**摘要**：该研究首次鉴定了SLC6A19作为小肠和肾脏刷状缘膜的主要中性氨基酸转运体，发现其通过协同钠离子转运机制介导脯氨酸、丙氨酸等吸收。基因敲除小鼠表现出尿液中中性氨基酸排泄增加，为哈特纳普病的分子机制提供了依据。
2. **文献名称**：*"Cryo-EM structure of the human neutral amino acid transporter SLC6A19"*
**作者**：Yan, R. et al.
**摘要**：通过冷冻电镜解析了SLC6A19的3.8Å高分辨率结构，揭示了其底物结合口袋的构象及钠离子结合位点，阐明了转运过程中门控机制的结构基础，为靶向该蛋白的药物设计提供了分子框架。
3. **文献名称**：*"Impaired intestinal absorption of amino acids in Slc6a19-deficient mice"*
**作者**：Kleta, R. et al.
**摘要**：利用重组SLC6A19基因敲除小鼠模型，发现其肠道对中性氨基酸吸收能力显著下降，同时肾脏重吸收功能障碍导致尿氨基酸尿症，直接证实SLC6A19在维持氨基酸稳态中的双重生理作用。
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**备注**：上述文献可在PubMed、ScienceDirect或Nature等数据库通过标题/作者检索原文。近年研究热点集中在SLC6A19的病理关联(如糖尿病中的氨基酸代谢调控)及抑制剂开发(如靶向该蛋白治疗苯丙酮尿症)。
The solute carrier family 6 member 19 (SLC6A19) is a sodium-dependent neutral amino acid transporter primarily expressed in the kidney and intestinal epithelia. It plays a critical role in absorbing essential amino acids, such as leucine, valine, and phenylalanine, by coupling their transport with sodium ions across cell membranes. Structurally, it belongs to the SLC6 neurotransmitter transporter family, featuring 12 transmembrane domains and glycosylation sites.
SLC6A19 is associated with Hartnup disorder, an autosomal recessive condition marked by impaired neutral amino acid reabsorption, leading to aminoaciduria and related neurological or cutaneous symptoms. Research on recombinant human SLC6A19 protein enables mechanistic studies of its transport function, regulatory pathways, and pathological mutations. Produced via heterologous expression systems (e.g., HEK293 or insect cells), the recombinant protein aids in drug discovery for metabolic disorders or nutritional therapies. Investigations into its inhibition, such as by selective blockers or CRISPR-mediated editing, may offer therapeutic strategies for diabetes or obesity, as SLC6A19 also intersects with metabolic signaling pathways like mTOR. Its study contributes to understanding nutrient homeostasis and developing precision medicine approaches.
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