| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC6A4 |
| Uniprot No | P31645 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-630 aa |
| 活性数据 | METTPLNSQKQLSACEDGEDCQENGVLQKVVPTPGDKVESGQISNGYSAVPSPGAGDDTRHSIPATTTTLVAELHQGERETWGKKVDFLLSVIGYAVDLGNVWRFPYICYQNGGGAFLLPYTIMAIFGGIPLFYMELALGQYHRNGCISIWRKICPIFKGIGYAICIIAFYIASYYNTIMAWALYYLISSFTDQLPWTSCKNSWNTGNCTNYFSEDNITWTLHSTSPAEEFYTRHVLQIHRSKGLQDLGGISWQLALCIMLIFTVIYFSIWKGVKTSGKVVWVTATFPYIILSVLLVRGATLPGAWRGVLFYLKPNWQKLLETGVWIDAAAQIFFSLGPGFGVLLAFASYNKFNNNCYQDALVTSVVNCMTSFVSGFVIFTVLGYMAEMRNEDVSEVAKDAGPSLLFITYAEAIANMPASTFFAIIFFLMLITLGLDSTFAGLEGVITAVLDEFPHVWAKRRERFVLAVVITCFFGSLVTLTFGGAYVVKLLEEYATGPAVLTVALIEAVAVSWFYGITQFCRDVKEMLGFSPGWFWRICWVAISPLFLLFIICSFLMSPPQLRLFQYNYPYWSIILGYCIGTSSFICIPTYIAYRLIITPGTFKERIIKSITPETPTEIPCGDIRLNAV |
| 分子量 | 96.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC6A4蛋白的3篇参考文献示例(注：内容为虚构，仅供参考)：
1. **文献名称**：*Expression and Functional Characterization of Recombinant Human SLC6A4 in Mammalian Cells*
**作者**：Chen et al.
**摘要**：本研究通过哺乳动物细胞系统成功表达并纯化功能性重组人SLC6A4蛋白，验证其对5-HT的高亲和力转运能力，为抑郁症相关药物筛选提供模型。
2. **文献名称**：*Cryo-EM Structure of the Human Serotonin Transporter SLC6A4*
**作者**：Wang & Zhang
**摘要**：利用冷冻电镜解析重组SLC6A4蛋白的原子级结构，揭示抗抑郁药物结合位点，阐明转运抑制剂的作用机制。
3. **文献名称**：*Optimization of SLC6A4 Protein Production in E. coli for Biochemical Studies*
**作者**：Kumar et al.
**摘要**：开发大肠杆菌中高效表达重组SLC6A4蛋白的方法，通过突变筛选提升可溶性表达水平，支持后续生化与药理研究。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“recombinant SLC6A4”、“human serotonin transporter expression”。
The serotonin transporter (SLC6A4/SERT) is a critical membrane protein responsible for reuptaking serotonin (5-hydroxytryptamine, 5-HT) from synaptic spaces into presynaptic neurons, thereby regulating serotonergic signaling and neurotransmitter homeostasis. Encoded by the *SLC6A4* gene, it plays a central role in mood, cognition, and emotional processing. Dysregulation of SERT is linked to neuropsychiatric disorders like depression, anxiety, and obsessive-compulsive disorder (OCD), making it a primary target for selective serotonin reuptake inhibitors (SSRIs), including fluoxetine and sertraline.
Recombinant human SLC6A4 protein is engineered using heterologous expression systems (e.g., mammalian cells, bacteria, or yeast) to study its structure, function, and interactions. Its production enables detailed analysis of ligand binding, transport mechanisms, and drug responses under controlled conditions. Structural studies, facilitated by recombinant SERT, have revealed insights into SSRIs' binding modes and the impact of genetic polymorphisms (e.g., 5-HTTLPR) on transporter efficiency. Researchers also use tagged or mutated versions to track cellular localization or assess functional variants.
Despite challenges in expressing and purifying this 12-transmembrane-domain protein, recombinant SLC6A4 remains vital for drug discovery and personalized medicine, particularly in tailoring antidepressants based on genetic profiles. Ongoing work aims to elucidate its conformational dynamics and develop novel therapeutics with fewer side effects.
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