| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC7A4 |
| Uniprot No | O43246 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-635 aa |
| 活性数据 | MARGLPTIASLARLCQKLNRLKPLEDSIMETSLRRCLSTLDLTLLGVGGMVGSGLYVLTGAVAKEVAGPAVLLSFGVAAVASLLAALCYAEFGARVPRTGSAYLFTYVSMGELWAFLIGWNVLLEYIIGGAAVARAWSGYLDSMFSHSIRNFTETHVGSWQVPLLGHYPDFLAAGIILLASAFVSCGARVSSWLNHTFSAISLLVILFIVILGFILAQPHNWSADEGGFAPFGFSGVMAGTASCFYAFVGFDVIAASSEEAQNPRRSVPLAIAISLAIAAGAYILVSTVLTLMVPWHSLDPDSALADAFYQRGYRWAGFIVAAGSICAMNTVLLSLLFSLPRIVYAMATDGLFFQVFAHVHPRTQVPVAGTLAFGLLTAFLALLLDLESLVQFLSLGTLLAYTFVATSIIVLRFQKSSPPSSPGPASPGPLTKQQSSFSDHLQLVGTVHASVPEPGELKPALRPYLGFLDGYSPGAVVTWALGVMLASAITIGCVLVFGNSTLHLPHWGYILLLLLTSVMFLLSLLVLGAHQQQYREDLFQIPMVPLIPALSIVLNICLMLKLSYLTWVRFSIWLLMGLAVYFGYGIRHSKENQRELPGLNSTHYVVFPRGSLEETVQAMQPPSQAPAQDPGHME |
| 分子量 | 96.25 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人SLC7A4蛋白的专门研究相对有限，目前公开发表的文献较少。以下提供部分可能与SLC家族或相关功能相关的参考信息(注意：SLC7A4研究较少，部分文献可能需结合家族背景分析)：
1. **文献名称**：*The SLC7 family of amino acid transporters: structure and function*
**作者**：Kanai Y. et al.
**摘要**：综述了SLC7溶质载体家族的结构与功能，提到SLC7A4作为潜在孤儿转运体，其具体底物尚未明确，可能与阳离子氨基酸转运相关，但缺乏重组蛋白表达的详细研究。
2. **文献名称**：*Genomic organization and functional characterization of the human SLC7A4 gene*
**作者**：Hatanaka T. et al.
**摘要**：通过基因定位和体外表达实验，发现SLC7A4在特定细胞系中的低表达水平，推测其在细胞增殖中发挥作用，但重组蛋白功能验证仍需深入。
3. **文献名称**：*Expression profiling of solute carrier gene families in the mouse brain*
**作者**：Jensen A.A. et al.
**摘要**：分析SLC家族在脑组织中的分布，提及SLC7A4在特定神经元中的转录本存在，提示其可能在神经递质运输中扮演角色，但缺乏重组蛋白水平的直接证据。
4. **文献名称**：*Systematic review of orphan solute carriers (SLCs) in drug discovery*
**作者**：César-Razquin A. et al.
**摘要**：讨论SLC7A4作为潜在药物靶点，指出其重组蛋白制备的难点及现有功能研究的缺口，呼吁开发特异性抗体或敲除模型推进研究。
**提示**：SLC7A4可能因研究不足被归类为“孤儿转运体”，建议结合SLC7家族其他成员(如SLC7A5/LAT1)的文献拓展分析，或关注最新预印本数据库(如bioRxiv)获取未发表数据。确认基因名称无误后，可尝试通过跨物种同源蛋白研究进行参考。
The human SLC7A4 protein, encoded by the *SLC7A4* gene, belongs to the solute carrier family 7 (SLC7), a group of membrane transporters involved in amino acid and nutrient transport. Specifically, SLC7A4 is categorized within the cationic amino acid transporter (CAT) subfamily, which facilitates the cellular uptake of cationic amino acids like arginine, lysine, and ornithine. Structurally, it is predicted to contain 12 transmembrane domains, a hallmark of SLC transporters, and may function as a heterodimer linked via a disulfide bond to a glycoprotein subunit (e.g., SLC3 family members) for proper membrane localization and activity.
SLC7A4 is notably expressed in immune-related tissues, such as the placenta, spleen, and peripheral blood leukocytes, suggesting a role in immune regulation or nutrient supply during immune responses. However, its biological functions remain less characterized compared to other SLC7 members like SLC7A5 (LAT1), which is linked to cancer metabolism. Limited studies indicate potential involvement in diseases, including immune disorders or cancers, though mechanistic insights are sparse. Recombinant SLC7A4 proteins are often generated for *in vitro* studies to elucidate transport kinetics, substrate specificity, and interactions with partner proteins. These tools aid in exploring its physiological relevance, therapeutic targeting potential, or diagnostic applications. Further research is needed to clarify its precise roles in health and disease.
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