| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC7A2 |
| Uniprot No | P52569 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-657 aa |
| 活性数据 | MIPCRAALTFARCLIRRKIVTLDSLEDTKLCRCLSTMDLIALGVGSTLGAGVYVLAGEVAKADSGPSIVVSFLIAALASVMAGLCYAEFGARVPKTGSAYLYTYVTVGELWAFITGWNLILSYVIGTSSVARAWSGTFDELLSKQIGQFLRTYFRMNYTGLAEYPDFFAVCLILLLAGLLSFGVKESAWVNKVFTAVNILVLLFVMVAGFVKGNVANWKISEEFLKNISASAREPPSENGTSIYGAGGFMPYGFTGTLAGAATCFYAFVGFDCIATTGEEVRNPQKAIPIGIVTSLLVCFMAYFGVSAALTLMMPYYLLDEKSPLPVAFEYVGWGPAKYVVAAGSLCALSTSLLGSMFPLPRILFAMARDGLLFRFLARVSKRQSPVAATLTAGVISALMAFLFDLKALVDMMSIGTLMAYSLVAACVLILRYQPGLSYDQPKCSPEKDGLGSSPRVTSKSESQVTMLQRQGFSMRTLFCPSLLPTQQSASLVSFLVGFLAFLVLGLSVLTTYGVHAITRLEAWSLALLALFLVLFVAIVLTIWRQPQNQQKVAFMVPFLPFLPAFSILVNIYLMVQLSADTWVRFSIWMAIGFLIYFSYGIRHSLEGHLRDENNEEDAYPDNVHAAAEEKSAIQANDHHPRNLSSPFIFHEKTSEF |
| 分子量 | 98.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC7A2蛋白的文献示例，内容基于文献领域常见研究方向构建，供参考：
---
1. **"Functional Characterization of Recombinant Human SLC7A2 as a Cationic Amino Acid Transporter"**
*Smith, J. et al.*
该研究通过克隆SLC7A2基因并在HEK293细胞中重组表达，证实其作为阳离子氨基酸转运体(如精氨酸、赖氨酸)的功能，并通过放射性标记实验分析其转运动力学特性。
2. **"SLC7A2-Mediated Arginine Transport in Macrophage Polarization and Nitric Oxide Production"**
*Lee, H. & Kim, M.*
研究利用SLC7A2重组蛋白及敲除模型，揭示其在巨噬细胞中调控精氨酸摄取、影响一氧化氮合成及M1/M2极化平衡的作用，为炎症反应机制提供新见解。
3. **"Crystallographic Analysis of Recombinant Human SLC7A2 Reveals Substrate Binding Sites"**
*Zhang, R. et al.*
通过杆状病毒-昆虫细胞系统表达并纯化SLC7A2蛋白，结合X射线晶体学解析其三维结构，阐明了底物结合口袋的关键氨基酸残基及转运机制。
4. **"SLC7A2 Gene Silencing Inhibits Tumor Growth via Depletion of Intracellular Arginine in Colorectal Cancer"**
*Wang, Y. et al.*
使用重组SLC7A2蛋白及siRNA技术，证明其通过调节癌细胞精氨酸代谢影响肿瘤增殖，提示其作为癌症治疗靶点的潜力。
---
**注意**：以上文献为示例，实际文献需通过PubMed/Google Scholar等平台检索。推荐检索关键词：
`SLC7A2 recombinant protein expression`、`SLC7A2 arginine transport`、`SLC7A2 structure-function`。若需真实文献，可进一步提供具体研究方向(如结构、疾病关联等)协助筛选。
Recombinant human SLC7A2 protein, encoded by the *SLC7A2* gene, belongs to the solute carrier family 7 (SLC7), a group of transmembrane transporters critical for amino acid homeostasis. SLC7A2. also known as CAT2 (cationic amino acid transporter 2), primarily facilitates the sodium-independent transport of cationic amino acids, such as arginine, lysine, and ornithine, across cellular membranes. This protein plays pivotal roles in physiological processes including nitric oxide synthesis (via arginine supply), immune cell function, and cell proliferation.
Structurally, SLC7A2 exists as a glycoprotein with 12 predicted transmembrane domains. It has two splice variants, CAT2A and CAT2B, differing in tissue expression and regulatory mechanisms. CAT2A is constitutively expressed in liver, skeletal muscle, and macrophages, while CAT2B is induced in activated immune cells, fibroblasts, and tumor-associated tissues under stress or inflammatory conditions.
Recombinant SLC7A2 is produced in heterologous expression systems (e.g., HEK293 or insect cells) for functional studies. Its dysregulation is linked to cardiovascular diseases, immune disorders, and cancer progression, particularly through modulating arginine metabolism in tumor microenvironments. Research applications include elucidating amino acid signaling pathways, drug-target interaction studies, and exploring therapeutic strategies targeting arginine deprivation in cancer. Its recombinant form enables precise biochemical characterization, aiding the development of inhibitors or modulators for clinical interventions.
×
