| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC6A7 |
| Uniprot No | Q99884 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-636 aa |
| 活性数据 | MKKLQGAHLRKPVTPDLLMTPSDQGDVDLDVDFAAHRGNWTGKLDFLLSCIGYCVGLGNVWRFPYRAYTNGGGAFLVPYFLMLAICGIPLFFLELSLGQFSSLGPLAVWKISPLFKGAGAAMLLIVGLVAIYYNMIIAYVLFYLFASLTSDLPWEHCGNWWNTELCLEHRVSKDGNGALPLNLTCTVSPSEEYWSRYVLHIQGSQGIGSPGEIRWNLCLCLLLAWVIVFLCILKGVKSSGKVVYFTATFPYLILLMLLVRGVTLPGAWKGIQFYLTPQFHHLLSSKVWIEAALQIFYSLGVGFGGLLTFASYNTFHQNIYRDTFIVTLGNAITSILAGFAIFSVLGYMSQELGVPVDQVAKAGPGLAFVVYPQAMTMLPLSPFWSFLFFFMLLTLGLDSQFAFLETIVTAVTDEFPYYLRPKKAVFSGLICVAMYLMGLILTTDGGMYWLVLLDDYSASFGLMVVVITTCLAVTRVYGIQRFCRDIHMMLGFKPGLYFRACWLFLSPATLLALLVYSIVKYQPSEYGSYRFPPWAELLGILMGLLSCLMIPAGMLVAVLREEGSLWERLQQASRPAMDWGPSLEENRTGMYVATLAGSQSPKPLMVHMRKYGGITSFENTAIEVDREIAEEEESMM |
| 分子量 | 97.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC6A7蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"Cloning and characterization of the human SLC6A7 gene encoding the human orthologue of the neutral amino acid transporter ASCT2"*
**作者**: Kristensen, A.S. et al.
**摘要**: 该研究报道了人类SLC6A7基因的克隆及其功能表征，揭示了其在HEK293细胞中的重组表达，并证明其作为钠离子依赖性中性氨基酸(如脯氨酸)转运体的功能，为后续神经递质调控研究奠定基础。
2. **文献名称**: *"Structural insights into the human SLC6A7 transporter by cryo-EM: implications for substrate binding and transport mechanism"*
**作者**: Farhan, S.M. et al.
**摘要**: 通过冷冻电镜技术解析了重组SLC6A7蛋白的三维结构，阐明其底物结合口袋的关键氨基酸残基及运输机制，揭示了该蛋白在脯氨酸转运中的构象变化及潜在药理学靶点。
3. **文献名称**: *"Proline transporter SLC6A7 is a novel marker for human colorectal cancer progression and survival"*
**作者**: Shishido, T. et al.
**摘要**: 研究在结肠癌细胞中重组表达了SLC6A7蛋白，证明其过表达促进肿瘤细胞增殖与迁移，并关联患者预后，提示其作为癌症治疗靶点的潜力。
(注：上述文献为虚拟示例，实际研究中建议通过PubMed或Web of Science检索最新论文。)
The solute carrier family 6 member 7 (SLC6A7) protein, also known as the high-affinity proline transporter, is a member of the sodium/chloride-dependent neurotransmitter transporter family. Primarily expressed in the central nervous system, SLC6A7 facilitates the reuptake of the non-essential amino acid L-proline, a putative neuromodulator in glutamatergic pathways. It localizes to presynaptic membranes and regulates synaptic proline concentration, influencing neurotransmission and synaptic plasticity. Structurally, it contains 12 transmembrane domains and conserved motifs critical for substrate binding and ion coupling. Recombinant human SLC6A7 protein is typically expressed in mammalian or insect cell systems (e.g., HEK293. Sf9) with epitope tags (His-tag, FLAG) for purification and detection. Its study aids in understanding proline's role in neurological processes and potential links to neuropsychiatric disorders. Dysregulation of SLC6A7 has been implicated in schizophrenia, depression, and anxiety in preclinical models, though its exact pathophysiological mechanisms remain under investigation. Current research focuses on characterizing transport kinetics, pharmacological profiling, and crystallographic studies to develop targeted therapies. Recombinant SLC6A7 serves as a critical tool for drug screening and mechanistic studies of neurotransmitter regulation.
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