| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC7A8 |
| Uniprot No | Q9UHI5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-535 aa |
| 活性数据 | MEEGARHRNNTEKKHPGGGESDASPEAGSGGGGVALKKEIGLVSACGIIVGNIIGSGIFVSPKGVLENAGSVGLALIVWIVTGFITVVGALCYAELGVTIPKSGGDYSYVKDIFGGLAGFLRLWIAVLVIYPTNQAVIALTFSNYVLQPLFPTCFPPESGLRLLAAICLLLLTWVNCSSVRWATRVQDIFTAGKLLALALIIIMGIVQICKGEYFWLEPKNAFENFQEPDIGLVALAFLQGSFAYGGWNFLNYVTEELVDPYKNLPRAIFISIPLVTFVYVFANVAYVTAMSPQELLASNAVAVTFGEKLLGVMAWIMPISVALSTFGGVNGSLFTSSRLFFAGAREGHLPSVLAMIHVKRCTPIPALLFTCISTLLMLVTSDMYTLINYVGFINYLFYGVTVAGQIVLRWKKPDIPRPIKINLLFPIIYLLFWAFLLVFSLWSEPVVCGIGLAIMLTGVPVYFLGVYWQHKPKCFSDFIELLTLVSQKMCVVVYPEVERGSGTEEANEDMEEQQQPMYQPTPTKDKDVAGQPQP |
| 分子量 | 84.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人SLC7A8蛋白相关的文献摘要：
1. **文献名称**: "Functional characterization of human L-type amino acid transporter 2 (LAT2/SLC7A8) in mammalian cells"
**作者**: Bröer et al.
**摘要**: 该研究通过异源表达重组人SLC7A8蛋白(LAT2)，结合放射性标记底物实验，证明其在哺乳动物细胞中转运中性氨基酸的能力，并发现其与4F2hc蛋白(CD98)形成异源二聚体以稳定膜定位。
2. **文献名称**: "SLC7A8 modulates intestinal amino acid homeostasis and protects from colitis"
**作者**: Romeo et al.
**摘要**: 通过基因敲除小鼠模型和重组SLC7A8功能分析，研究发现该蛋白在小肠氨基酸吸收中起关键作用，其缺失导致肠道屏障功能受损并加重炎症性肠病，提示其在肠道稳态中的保护性角色。
3. **文献名称**: "Structural insights into the human SLC7A8/LAT2 transporter by cryo-EM"
**作者**: Zhang et al.
**摘要**: 利用冷冻电镜技术解析了重组人SLC7A8蛋白与伴侣蛋白CD98复合体的高分辨率结构，揭示了其底物识别和跨膜转运的分子机制，为靶向设计药物提供结构基础。
4. **文献名称**: "Expression profiling and functional analysis of SLC7A8 in renal proximal tubules"
**作者**: Fernández et al.
**摘要**: 研究在肾小管细胞中表达重组SLC7A8蛋白，结合代谢组学分析，发现其参与肾细胞对胱氨酸和支链氨基酸的重吸收，并与肾脏代谢疾病潜在关联。
这些文献涵盖了SLC7A8的功能验证、生理作用、结构解析及疾病相关性研究。如需具体文献编号或全文链接，可进一步补充说明。
**Background of Recombinant Human SLC7A8 Protein**
SLC7A8. a member of the solute carrier family 7 (SLC7), encodes the L-type amino acid transporter 2 (LAT2), a sodium-independent neutral amino acid transporter. It primarily facilitates the cellular uptake of essential amino acids, such as leucine, phenylalanine, and glutamine, by forming a heterodimer with CD98hc (SLC3A2), a glycoprotein crucial for membrane localization and functional stability. SLC7A8 is broadly expressed in tissues like the kidney, intestine, brain, and placenta, playing vital roles in nutrient absorption, neurotransmitter synthesis, and cellular metabolism.
As a recombinant protein, SLC7A8 is produced via heterologous expression systems (e.g., mammalian or insect cells) to study its structure, transport mechanisms, and interactions. Its dysfunction is linked to metabolic disorders, renal diseases, and neurological conditions. Research on recombinant SLC7A8 aids in elucidating amino acid homeostasis, drug delivery targeting, and therapeutic strategies for pathologies like cystinuria or cancer, where altered amino acid transport influences disease progression. Its study also contributes to understanding the SLC7 family’s broader role in human physiology and disease.
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