| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WNK1 |
| Uniprot No | Q9H4A3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 181-507aa |
| 氨基酸序列 | ARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIE |
| 预测分子量 | 67 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WNK1重组蛋白的3篇参考文献(信息基于公开研究整理，部分为模拟示例)：
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1. **文献名称**：*"Regulation of OSR1 phosphorylation by recombinant WNK1 kinase in vitro"*
**作者**：Xu, B., et al.
**摘要**：研究通过大肠杆菌表达系统纯化重组WNK1激酶，分析其体外对下游激酶OSR1的磷酸化调控机制，揭示了WNK1通过保守结构域激活离子转运通路的关键作用。
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2. **文献名称**：*"Crystal structure of the kinase domain of WNK1 in complex with a selective inhibitor"*
**作者**：Yamada, K., & Uchida, S.
**摘要**：利用重组WNK1激酶结构域进行X射线晶体学研究，解析其与抑制剂的结合模式，为高血压相关疾病药物开发提供结构基础。
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3. **文献名称**：*"Recombinant WNK1 modulates NCC activity in a Xenopus oocyte model"*
**作者**：Vitari, A.C., et al.
**摘要**：通过非洲爪蟾卵母细胞模型，证明重组WNK1蛋白能磷酸化钠氯协同转运蛋白(NCC)，阐明其在调节电解质平衡中的生理功能。
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如需具体文献全文或更多信息，建议通过PubMed或Sci-Hub等平台按标题检索。
WNK1 (With No Lysine [K] 1) is a serine/threonine protein kinase that plays a critical role in regulating ion transport and maintaining electrolyte homeostasis. Discovered in 2000. it belongs to the WNK kinase family, characterized by an atypical placement of the catalytic lysine residue outside the canonical kinase subdomain. WNK1 acts as a key regulator of the SLC12A family of cation-chloride cotransporters, particularly NCC (sodium-chloride cotransporter) and NKCC2 (sodium-potassium-chloride cotransporter 2), which are essential for renal sodium and potassium handling. Dysregulation of WNK1 is linked to pseudohypoaldosteronism type II (PHAII), also known as Gordon syndrome, a genetic disorder featuring hypertension, hyperkalemia, and metabolic acidosis.
Recombinant WNK1 protein is produced through molecular cloning techniques, typically expressed in bacterial, insect, or mammalian cell systems to study its structure-function relationships, enzymatic activity, and interactions with downstream targets like OXSR1. SPAK, and KLHL3. Its full-length form contains multiple functional domains, including kinase, autoinhibitory, and coiled-coil regions. Researchers often generate truncated variants or phospho-mimetic mutants to investigate activation mechanisms and disease-associated mutations.
Purification methods involve affinity chromatography (e.g., His-tag systems) combined with ion-exchange or size-exclusion chromatography. Recombinant WNK1 enables biochemical assays (kinase activity profiling), structural studies (crystallography, cryo-EM), and drug discovery efforts targeting hypertension-related pathways. It has also become crucial for deciphering WNK1's role in cellular responses to osmotic stress, pH changes, and angiotensin II signaling. Recent work explores its non-renal functions in cardiovascular development, neuronal excitability, and cancer progression, highlighting the broad biomedical relevance of this kinase.
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