| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX30 |
| Uniprot No | O94993 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-753 aa |
| 活性数据 | MERARPEPPP QPRPLRPAPP PLPVEGTSFW AAAMEPPPSS PTLSAAASAT LASSCGEAVA SGLQPAVRRL LQVKPEQVLL LPQPQAQNEE AAASSAQARL LQFRPDLRLL QPPTASDGAT SRPELHPVQP LALHVKAKKQ KLGPSLDQSV GPRGAVETGP RASRVVKLEG PGPALGYFRG DEKGKLEAEE VMRDSMQGGA GKSPAAIREG VIKTEEPERL LEDCRLGAEP ASNGLVHGSA EVILAPTSGA FGPHQQDLRI PLTLHTVPPG ARIQFQGAPP SELIRLTKVP LTPVPTKMQS LLEPSVKIET KDVPLTVLPS DAGIPDTPFS KDRNGHVKRP MNAFMVWARI HRPALAKANP AANNAEISVQ LGLEWNKLSE EQKKPYYDEA QKIKEKHREE FPGWVYQPRP GKRKRFPLSV SNVFSGTTQN IISTNPTTVY PYRSPTYSVV IPSLQNPITH PVGETSPAIQ LPTPAVQSPS PVTLFQPSVS SAAQVAVQDP SLPVYPALPP QRFTGPSQTD THQLHSEATH TVKQPTPVSL ESANRISSSA STAHARFATS TIQPPREYSS VSPCPRSAPI PQASPIPHPH VYQPPPLGHP ATLFGTPPRF SFHHPYFLPG PHYFPSSTCP YSRPPFGYGN FPSSMPECLS YYEDRYPKHE GIFSTLNRDY SFRDYSSECT HSENSRSCEN MNGTSYYNSH SHSGEENLNP VPQLDIGTLE NVFTAPTSTP SSIQQVNVTD SDEEEEEKVL RDL |
| 分子量 | 81.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SOX30蛋白的3条参考文献示例(基于公开研究主题模拟概括，具体文献需依据实际发表内容为准)：
1. **文献名称**: "SOX30 regulates spermatogenesis through transcriptional activation of testis-specific genes"
**作者**: Zhang Y, et al.
**摘要**: 首次揭示SOX30蛋白在哺乳动物睾丸发育中的关键作用，通过重组人SOX30蛋白的体外实验证实其直接结合特定基因启动子区，调控精子形成相关基因的转录。
2. **文献名称**: "Recombinant human SOX30 induces apoptosis in lung cancer cells via epigenetic modulation"
**作者**: Li H, et al.
**摘要**: 研究重组人SOX30蛋白在肺癌细胞中的功能，证明其通过去甲基化修饰抑制肿瘤增殖并诱导凋亡，提示其作为潜在抗癌靶点。
3. **文献名称**: "Prokaryotic expression and purification of human SOX30 transcription factor"
**作者**: Wang X, et al.
**摘要**: 报道了一种高效的大肠杆菌表达系统制备重组人SOX30蛋白的方法，优化纯化条件后获得高纯度活性蛋白，为后续功能研究提供可靠工具。
*注：若需真实文献，建议通过PubMed或Sci-Hub输入关键词“SOX30 recombinant”检索具体文章。部分研究可能集中在SOX30基因功能而非重组蛋白制备，需进一步筛选。*
The SOX30 protein belongs to the SOX (SRY-related HMG-box) family of transcription factors, characterized by a conserved high-mobility group (HMG) DNA-binding domain. SOX30. a member of the Group H SOX proteins, is distinct for its specific expression pattern and role in germ cell development. Initially identified in mice, Sox30 is predominantly expressed in the testis, particularly during late spermatogenesis, suggesting its critical involvement in sperm maturation. Studies in mice have shown that SOX30 deficiency leads to male infertility due to disrupted spermiation and abnormal chromatin condensation in elongating spermatids.
In humans, SOX30 is similarly testis-enriched, though its functional details remain less explored compared to other SOX members. It regulates target genes by binding to conserved DNA motifs, potentially interacting with chromatin remodelers to modulate transcriptional programs during spermatogenesis. Emerging evidence also links SOX30 to epigenetic regulation, including histone modification, to ensure proper chromatin packaging in developing sperm.
Recombinant human SOX30 protein is widely used to study its biochemical properties, DNA-binding specificity, and interactions with co-regulators in vitro. Despite progress, many aspects of SOX30 remain unclear, such as its broader tissue distribution, regulatory networks, and potential roles in non-reproductive systems. Further research is needed to clarify its mechanisms in humans and its implications in male infertility or related disorders.
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