| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX15 |
| Uniprot No | O60248 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-233 aa |
| 活性数据 | MALPGSSQDQ AWSLEPPAAT AAASSSSGPQ EREGAGSPAA PGTLPLEKVK RPMNAFMVWS SAQRRQMAQQ NPKMHNSEIS KRLGAQWKLL DEDEKRPFVE EAKRLRARHL RDYPDYKYRP RRKAKSSGAG PSRCGQGRGN LASGGPLWGP GYATTQPSRG FGYRPPSYST AYLPGSYGSS HCKLEAPSPC SLPQSDPRLQ GELLPTYTHY LPPGSPTPYN PPLAGAPMPL THL |
| 分子量 | 25.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SOX15蛋白的参考文献列表，包含文献名称、作者及简要摘要内容：
1. **文献名称**: *"Production and characterization of recombinant human SOX15 protein for stem cell differentiation studies"*
**作者**: Lee, J. et al.
**摘要**: 该研究通过大肠杆菌表达系统成功制备了重组人SOX15蛋白，验证了其DNA结合活性，并证明其可增强小鼠胚胎干细胞的神经分化效率。
2. **文献名称**: *"SOX15 regulates skeletal muscle regeneration via direct transcriptional activation of satellite cell-specific genes"*
**作者**: Tanaka, M. et al.
**摘要**: 研究发现重组人SOX15蛋白通过激活肌卫星细胞基因(如MyoD和Pax7)，在小鼠模型中促进骨骼肌损伤修复，揭示了其在肌肉再生中的关键作用。
3. **文献名称**: *"Structural insights into the DNA-binding specificity of human SOX15 transcription factor"*
**作者**: Zhang, R. et al.
**摘要**: 通过X射线晶体学解析重组人SOX15蛋白的HMG结构域与DNA复合体结构，揭示其结合特定DNA序列(CAAAG)的分子机制，为靶向设计提供依据。
4. **文献名称**: *"SOX15 modulates Wnt/β-catenin signaling in colorectal cancer by repressing CD44 expression"*
**作者**: Chen, L. et al.
**摘要**: 研究利用重组SOX15蛋白验证其通过抑制Wnt信号通路靶基因CD44的表达，抑制结直肠癌细胞增殖和迁移，提示其作为肿瘤抑制因子的潜力。
**注**：以上文献信息为模拟生成，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议使用关键词“recombinant SOX15”或“human SOX15 protein”结合“expression”、“function”等进行精确查询。
Sox15 protein, a member of the Sox (SRY-related HMG box) transcription factor family, plays a critical role in cellular differentiation and tissue development. Encoded by the *SOX15* gene in humans, it belongs to the SoxD subgroup and shares a conserved HMG (high-mobility group) DNA-binding domain, enabling sequence-specific interactions with regulatory regions of target genes. Though less studied than other Sox members, Sox15 is implicated in myogenesis, neurogenesis, and stem cell pluripotency. It is highly expressed in embryonic stem cells and during early development, suggesting a role in maintaining cellular plasticity. Studies reveal its involvement in muscle regeneration by cooperating with MyoD to activate myogenic differentiation.
Recombinant human Sox15 protein is typically produced using bacterial or mammalian expression systems, purified via affinity chromatography for functional studies. Its truncated or tagged variants (e.g., GST, His-tag) are utilized to explore DNA-binding specificity, protein-protein interactions, or regulatory mechanisms in vitro. Research applications span developmental biology, regenerative medicine, and disease modeling, particularly in disorders linked to dysregulated differentiation pathways, such as muscular dystrophies or cancers. Emerging evidence also highlights its potential in reprogramming somatic cells and modulating stem cell fate. Despite its niche status, Sox15’s dual regulatory roles—activating lineage-specific genes while suppressing pluripotency factors—make it a compelling target for understanding cell fate decisions and therapeutic development.
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