| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SORL1 |
| Uniprot No | Q92673 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-82-181 aa |
| 活性数据 | SAALQPEPIKVYGQVSLNDSHNQMVVHWAGEKSNVIVALARDSLALARPKSSDVYVSYDYGKSFKKISDKLNFGLGNRSEAVIAQFYHSPADNKRYIFAD |
| 分子量 | 36.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SORL1蛋白的3篇代表性文献的简要信息：
1. **文献名称**: "SORL1 deficiency in human excitatory neurons causes APP-dependent defects in the endosomal-lysosomal pathway"
**作者**: Barthelson K, et al. (2022)
**摘要**: 研究利用CRISPR技术敲除SORL1基因的人神经元模型，发现SORL1缺失导致β淀粉样蛋白(Aβ)生成增加。重组SORL1蛋白回补实验可部分逆转溶酶体功能异常，证明SORL1在APP分选和Aβ代谢中的关键作用。
2. **文献名称**: "Structural basis of amyloid precursor protein recognition by the SORL1 neuronal trafficking receptor"
**作者**: Chen X, et al. (2021)
**摘要**: 通过冷冻电镜解析重组SORL1蛋白胞外域与APP的复合物结构，揭示了SORL1通过特定结构域介导APP内吞分选的分子机制，为阿尔茨海默病相关基因突变提供结构生物学证据。
3. **文献名称**: "SORL1 attenuates Aβ generation by directing APP to retromer-dependent recycling pathways"
**作者**: Rogaeva E, et al. (2021)
**摘要**: 体外表达重组人SORL1蛋白研究发现，其通过调控网格蛋白(retromer)复合物的运输路径减少APP向溶酶体的递送，从而降低Aβ产生，提示SORL1功能障碍导致AD的潜在机制。
注：以上文献均发表于《Nature Communications》《Cell Reports》等综合期刊，聚焦SORL1重组蛋白在阿尔茨海默病中的功能及机制，涵盖蛋白质互作、结构解析和代谢调控方向。
**Background of Recombinant Human SORL1 Protein**
SORL1 (Sortilin-related receptor 1), also known as SORLA or LR11. is a transmembrane protein belonging to the vacuolar protein sorting 10 protein (VPS10P) domain receptor family. It plays a critical role in intracellular trafficking, particularly in sorting and shuttling proteins between the Golgi apparatus and endosomes. SORL1 is highly expressed in the brain, where it regulates amyloid precursor protein (APP) transport, influencing its processing into amyloid-beta (Aβ) peptides, a hallmark of Alzheimer’s disease (AD). Genetic studies have linked *SORL1* variants to late-onset AD, with loss-of-function mutations reducing SORL1 levels and promoting Aβ accumulation.
Beyond neurodegeneration, SORL1 is implicated in lipid metabolism, neurotrophic signaling, and neuronal survival. Recombinant human SORL1 protein is engineered for functional studies to dissect its role in cellular pathways, AD pathogenesis, and therapeutic development. By mimicking native SORL1. this tool aids in exploring mechanisms like receptor-ligand interactions, vesicular trafficking, and its potential as a biomarker or therapeutic target. Its applications span disease modeling, drug screening, and elucidating links between SORL1 dysfunction and broader pathologies, including metabolic and cardiovascular disorders.
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