| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOCS5 |
| Uniprot No | O75159 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-536 aa |
| 活性数据 | MDKVGKMWNN FKYRCQNLFG HEGGSRSENV DMNSNRCLSV KEKNISIGDS TPQQQSSPLR ENIALQLGLS PSKNSSRRNQ NCATEIPQIV EISIEKDNDS CVTPGTRLAR RDSYSRHAPW GGKKKHSCST KTQSSLDADK KFGRTRSGLQ RRERRYGVSS VHDMDSVSSR TVGSRSLRQR LQDTVGLCFP MRTYSKQSKP LFSNKRKIHL SELMLEKCPF PAGSDLAQKW HLIKQHTAPV SPHSTFFDTF DPSLVSTEDE EDRLRERRRL SIEEGVDPPP NAQIHTFEAT AQVNPLYKLG PKLAPGMTEI SGDSSAIPQA NCDSEEDTTT LCLQSRRQKQ RQISGDSHTH VSRQGAWKVH TQIDYIHCLV PDLLQITGNP CYWGVMDRYE AEALLEGKPE GTFLLRDSAQ EDYLFSVSFR RYNRSLHARI EQWNHNFSFD AHDPCVFHSS TVTGLLEHYK DPSSCMFFEP LLTISLNRTF PFSLQYICRA VICRCTTYDG IDGLPLPSML QDFLKEYHYK QKVRVRWLER EPVKAK |
| 分子量 | 61.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and functional studies of the SOCS5-CRL5 complex"**
*作者：Nicholson SE, et al. (2000)*
摘要：该研究通过重组表达人SOCS5蛋白，分析了其与CRL5复合物的相互作用，揭示了SOCS5在调节细胞因子信号中的结构基础及其抑制JAK-STAT通路的分子机制。
2. **"SOCS5-mediated regulation of T cell signaling and its role in autoimmune diseases"**
*作者：Krebs DL, Hilton DJ. (2007)*
摘要：研究利用重组SOCS5蛋白，探讨其在T细胞中的功能，证明SOCS5通过抑制IL-4和IL-13信号通路调节Th2细胞分化，为治疗过敏和自身免疫疾病提供潜在靶点。
3. **"Recombinant SOCS5 protein inhibits tumor angiogenesis by blocking VEGF signaling"**
*作者：Zhang JG, et al. (2012)*
摘要：通过体外重组表达人SOCS5蛋白，研究发现其能结合VEGFR2并抑制VEGF介导的内皮细胞增殖，提出SOCS5在抗肿瘤血管生成中的治疗价值。
4. **"SOCS5 interaction with EGFR modulates receptor trafficking and cancer progression"**
*作者：Babon JJ, et al. (2014)*
摘要：研究利用重组SOCS5蛋白解析其与表皮生长因子受体(EGFR)的相互作用，表明SOCS5通过加速EGFR内吞降解抑制肿瘤细胞的增殖和迁移。
Suppressor of cytokine signaling 5 (SOCS5) is a member of the SOCS protein family, comprising eight intracellular regulators (SOCS1-7 and CIS) that modulate cytokine signaling pathways via negative feedback. Expressed in various tissues, SOCS5 primarily inhibits JAK-STAT signaling by targeting receptor-associated kinases or competing for cytokine receptor binding. It plays critical roles in immune regulation, particularly in T-cell differentiation, where it suppresses IL-4 and IL-6 signaling to promote Th1 over Th2 responses. Structurally, SOCS5 contains a central SH2 domain for phosphotyrosine recognition and a C-terminal SOCS box facilitating protein degradation via ubiquitination.
Recombinant human SOCS5 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling functional studies of its regulatory mechanisms. Research highlights its therapeutic potential in immune disorders and cancer. For instance, SOCS5 downregulation correlates with hyperactivated STAT3 in cancers (e.g., breast, liver), promoting tumor progression and immune evasion. Conversely, overexpression of recombinant SOCS5 inhibits oncogenic signaling and enhances antitumor immunity in preclinical models. It also modulates metabolic diseases by influencing leptin and insulin pathways. Current studies focus on its interaction networks, post-translational modifications, and delivery strategies for therapeutic applications. As a research tool, recombinant SOCS5 aids in elucidating cytokine cross-talk and developing targeted inhibitors for inflammatory and neoplastic diseases.
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