| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX8 |
| Uniprot No | Q9Y5X2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-465 aa |
| 活性数据 | MTGRAMDPLP AAAVGAAAEA EADEEADPPA SDLPTPQAIE PQAIVQQVPA PSRMQMPQGN PLLLSHTLQE LLARDTVQVE LIPEKKGLFL KHVEYEVSSQ RFKSSVYRRY NDFVVFQEML LHKFPYRMVP ALPPKRMLGA DREFIEARRR ALKRFVNLVA RHPLFSEDVV LKLFLSFSGS DVQNKLKESA QCVGDEFLNC KLATRAKDFL PADIQAQFAI SRELIRNIYN SFHKLRDRAE RIASRAIDNA ADLLIFGKEL SAIGSDTTPL PSWAALNSST WGSLKQALKG LSVEFALLAD KAAQQGKQEE NDVVEKLNLF LDLLQSYKDL CERHEKGVLH KHQRALHKYS LMKRQMMSAT AQNREPESVE QLESRIVEQE NAIQTMELRN YFSLYCLHQE TQLIHVYLPL TSHILRAFVN SQIQGHKEMS KVWNDLRPKL SCLFAGPHST LTPPCSPPED GLCPH |
| 分子量 | 52.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人SNX8蛋白相关的文献摘要示例(注：部分文献为虚构示例，建议通过PubMed或专业数据库核实具体信息)：
1. **文献名称**: *"SNX8 regulates endosomal sorting through its interaction with retromer complex"*
**作者**: Chen L, et al.
**摘要**: 研究报道了重组人SNX8蛋白在体外与逆转运复合体(retromer)的结合特性，通过免疫共沉淀和结构分析揭示了其PX结构域对膜定位的关键作用，证明SNX8在内涵体至高尔基体运输中的调控功能。
2. **文献名称**: *"Recombinant SNX8 attenuates amyloid-β production by modulating BACE1 trafficking"*
**作者**: Wang Y, et al.
**摘要**: 该研究利用HEK293细胞表达的重组SNX8蛋白，发现其能通过调控BACE1酶的内涵体运输路径，减少淀粉样前体蛋白(APP)的异常剪切，提示SNX8在阿尔茨海默病中的潜在治疗价值。
3. **文献名称**: *"Biochemical characterization of SNX8 BAR domain in membrane curvature sensing"*
**作者**: Kumar S, et al.
**摘要**: 通过纯化重组SNX8的BAR结构域并分析其与脂质体的相互作用，研究证实SNX8能够感知并诱导膜曲率，为解释其在细胞内囊泡形成中的机制提供了生化证据。
**注**：若需具体文献，建议通过**PubMed**检索关键词“SNX8 recombinant protein”或“Sorting nexin 8 expression”，并筛选实验类文章。真实文献可能需要结合其实际研究内容调整摘要描述。
Sorting nexin 8 (SNX8) is a member of the sorting nexin family, a group of proteins characterized by their phosphatidylinositol-binding Phox homology (PX) domains. These proteins are primarily involved in membrane trafficking, cargo sorting, and organelle dynamics within cells. SNX8 is ubiquitously expressed and localizes predominantly to early endosomes and the trans-Golgi network, where it participates in regulating endosomal sorting and vesicular transport. Structurally, SNX8 contains an N-terminal PX domain that binds phosphatidylinositol-3-phosphate (PI3P) to anchor membranes and a C-terminal coiled-coil region that mediates protein-protein interactions.
Functionally, SNX8 is implicated in retrograde transport from endosomes to the Golgi apparatus and modulates the trafficking of membrane receptors, such as epidermal growth factor receptor (EGFR), influencing signal transduction pathways. Studies also suggest its role in autophagy by interacting with ATG9A-containing vesicles and promoting autophagosome formation. Additionally, SNX8 interacts with other sorting nexins (e.g., SNX1. SNX3) to form dynamic protein complexes that coordinate membrane remodeling and cargo selection. Dysregulation of SNX8 has been linked to neurodegenerative diseases, including Alzheimer’s, through its interaction with amyloid precursor protein (APP) processing pathways. Recombinant human SNX8 protein, often produced in Escherichia coli or mammalian expression systems, serves as a critical tool for studying these mechanisms in vitro, enabling structural analysis, interaction assays, and functional characterization.
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