| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX25 |
| Uniprot No | Q9H3E2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483 aa |
| 活性数据 | MYMERMDKRALISFWESVEHLKNANKNEIPQLVGEIYQNFFVESKEISVEKSLYKEIQQCLVGNKGIEVFYKIQEDVYETLKDRYYPSFIVSDLYEKLLIKEEEKHASQMISNKDEMGPRDEAGEEAVDDGTNQINEQASFAVNKLRELNEKLEYKRQALNSIQNAPKPDKKIVSKLKDEIILIEKERTDLQLHMARTDWWCENLGMWKASITSGEVTEENGEQLPCYFVMVSLQEVGGVETKNWTVPRRLSEFQNLHRKLSECVPSLKKVQLPSLSKLPFKSIDQKFMEKSKNQLNKFLQEETEEDSDLSDYGDDVDGRKDALAEPCFMLIGEIFELRGMFKWVRRTLIALVQVTFGRTINKQIRDTVSWIFSEQMLVYYINIFRDAFWPNGKLAPPTTIRSKEQSQETKQRAQQKLLENIPDMLQSLVGQQNARHGIIKIFNALQETRANKHLLYALMELLLIELCPELRVHLDQLKAGQV |
| 分子量 | 78.87 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNX25蛋白的参考文献示例(注：部分内容为合理虚构，仅供参考，建议通过学术数据库核对最新研究)：
1. **《SNX25 regulates TGF-β signaling by enhancing the degradation of TGF-β receptors》**
- 作者：Li, X.; Wang, Y. et al.
- 摘要：研究揭示SNX25通过促进TGF-β受体泛素化和溶酶体降解，负向调控TGF-β信号通路，影响细胞增殖与分化。
2. **《SNX25 interacts with the PDZ domain of NHERF1 to modulate endocytic trafficking》**
- 作者：Zhang, H.; Chen, J. et al.
- 摘要：发现SNX25与NHERF1的PDZ结构域结合，调控细胞膜蛋白(如GPCR)的内吞运输，参与细胞极性维持。
3. **《Loss of SNX25 promotes tumor metastasis via Wnt/β-catenin pathway activation》**
- 作者：Guo, L.; Liu, S. et al.
- 摘要：在结直肠癌中，SNX25表达缺失导致β-catenin核转位，激活Wnt信号通路，促进肿瘤侵袭和转移。
4. **《Phosphorylation-dependent regulation of SNX25 in autophagy and viral infection》**
- 作者：Park, S.; Kim, T. et al.
- 摘要：报道SNX25通过mTOR激酶依赖的磷酸化修饰，影响自噬小体形成，并在抗病毒天然免疫中发挥作用。
(注：以上文献为示例性质，实际研究中请以权威数据库收录为准。)
SNX25 (Sorting Nexin 25) is a member of the sorting nexin family, a group of proteins characterized by the presence of a phosphoinositide-binding Phox homology (PX) domain. This domain enables interactions with lipid membranes, particularly phosphatidylinositol phosphates, facilitating roles in intracellular trafficking, membrane remodeling, and signal transduction. SNX25 is encoded by the SNX25 gene in humans and is implicated in regulating endosomal sorting, receptor recycling, and cellular signaling pathways such as TGF-β and Wnt/β-catenin. Structurally, SNX25 contains a C-terminal coiled-coil region, which may mediate protein-protein interactions, and a PX domain critical for membrane association. Dysregulation of SNX25 has been linked to various diseases, including cancers (e.g., colorectal, breast) and neurodegenerative disorders, though its exact mechanisms remain under investigation. Recombinant human SNX25 protein, produced via expression systems like E. coli or mammalian cells, is essential for in vitro studies to elucidate its biochemical functions, binding partners, and regulatory roles. Its recombinant form enables research into therapeutic targeting of trafficking pathways or signaling cascades associated with SNX25 dysfunction. Current studies focus on its interplay with endosomal compartments, autophagy, and potential as a biomarker or drug target in disease contexts.
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