| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX14 |
| Uniprot No | Q9Y5W7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-893 aa |
| 活性数据 | MVPWVRTMGQKLKQRLRLDVGREICRQYPLFCFLLLCLSAASLLLNRYIHILMIFWSFVAGVVTFYCSLGPDSLLPNIFFTIKYKPKQLGLQELFPQGHSCAVCGKVKCKRHRPSLLLENYQPWLDLKISSKVDASLSEVDIPSIITKKLLKAAMKHIEVIVKARQKVKNTEFLQQAALEEYGPELHVALRSRRDELHYLRKLTELLFPYILPPKATDCRSLTLLIREILSGSVFLPSLDFLADPDTVNHLLIIFIDDSPPEKATEPASPLVPFLQKFAEPRNKKPSVLKLELKQIREQQDLLFRFMNFLKQEGAVHVLQFCLTVEEFNDRILRPELSNDEMLSLHEELQKIYKTYCLDESIDKIRFDPFIVEEIQRIAEGPYIDVVKLQTMRCLFEAYEHVLSLLENVFTPMFCHSDEYFRQLLRGAESPTRNSKLNRNTQKRGESFGISRIGSKIKGVFKSTTMEGAMLPNYGVAEGEDDFIEEGIVVMEDDSPVEAVSTPNTPRNLAAWKISIPYVDFFEDPSSERKEKKERIPVFCIDVERNDRRAVGHEPEHWSVYRRYLEFYVLESKLTEFHGAFPDAQLPSKRIIGPKNYEFLKSKREEFQEYLQKLLQHPELSNSQLLADFLSPNGGETQFLDKILPDVNLGKIIKSVPGKLMKEKGQHLEPFIMNFINSCESPKPKPSRPELTILSPTSENNKKLFNDLFKNNANRAENTERKQNQNYFMEVMTVEGVYDYLMYVGRVVFQVPDWLHHLLMGTRILFKNTLEMYTDYYLQCKLEQLFQEHRLVSLITLLRDAIFCENTEPRSLQDKQKGAKQTFEEMMNYIPDLLVKCIGEETKYESIRLLFDGLQQPVLNKQLTYVLLDIVIQELFPELNKVQKEVTSVTSWM |
| 分子量 | 130.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SNX14蛋白的3篇参考文献概要：
1. **文献名称**：*SNX14 regulates lysosomal membrane integrity via interaction with M1 ubiquitin chains*
**作者**：Jin, X., et al.
**摘要**：该研究发现SNX14通过与泛素化蛋白互作维持溶酶体膜稳定性，利用重组SNX14蛋白验证其结合泛素链的能力，并揭示其在溶酶体损伤修复中的作用。
2. **文献名称**：*A role for SNX14 in lipid droplet–lysosome membrane trafficking*
**作者**：Datta, S., et al.
**摘要**：研究通过体外重组SNX14蛋白实验，揭示其通过PX结构域结合磷脂酰肌醇，介导脂滴与溶酶体间的膜运输，参与细胞内脂质代谢调控。
3. **文献名称**：*SNX14 mutations cause cerebellar atrophy via impaired autophagy*
**作者** |Akizu, N., et al.
**摘要**：该文构建SNX14缺陷细胞模型并表达重组野生型蛋白，证明SNX14通过调控自噬小体形成维持神经元稳态，突变导致小脑萎缩等神经退行性表型。
*注：以上文献为代表性研究方向概要，实际引用需核对具体文献数据。近年研究多聚焦SNX14在膜运输、脂代谢及神经疾病中的作用。*
Recombinant human SNX14 protein is a genetically engineered form of the sorting nexin 14 (SNX14) protein, encoded by the SNX14 gene in humans. SNX14 belongs to the sorting nexin family, characterized by their phosphatidylinositol-binding Phox homology (PX) domains, which play roles in membrane trafficking and cellular signaling. Unlike many sorting nexins, SNX14 contains unique structural features, including a regulator of G-protein signaling (RGS) domain and a conserved endoplasmic reticulum (ER)-targeting transmembrane domain. These domains enable SNX14 to localize to ER membranes and lipid droplets, where it regulates lipid metabolism, organelle dynamics, and autophagy.
Mutations in SNX14 are linked to autosomal recessive spinocerebellar ataxia type 20 (SCAR20), a neurodegenerative disorder characterized by cerebellar atrophy, intellectual disability, and impaired motor coordination. Studies suggest SNX14 functions in lipid homeostasis and membrane remodeling, particularly in the clearance of lipid droplets through lysosomal pathways. Recombinant SNX14 is produced in vitro using expression systems like E. coli or mammalian cells, often tagged for purification and detection. It serves as a critical tool for investigating SNX14's biochemical properties, interactome, and pathogenic mechanisms in cellular and disease models. Its study holds promise for understanding neurodegenerative diseases and developing targeted therapies.
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