| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX12 |
| Uniprot No | Q9UMY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-172 aa |
| 活性数据 | SDTAVADTRRLNSKPQDLTDAYGPPSNFLEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRGDEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEAIDRNYVPGKSLAVSCPGWSAVA |
| 分子量 | 24.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SNX12蛋白的参考文献(示例为假设性文献，仅供参考)：
---
1. **文献名称**：*"Sorting nexin 12 interacts with the β1-adrenergic receptor and regulates its lysosomal degradation"*
**作者**：Smith A, Jones B, et al.
**摘要**：该研究通过重组人SNX12蛋白的体外结合实验，揭示了SNX12与β1-肾上腺素受体的相互作用，并证明其通过溶酶体途径调控受体降解，提示SNX12在GPCR信号调控中的潜在作用。
2. **文献名称**：*"Structural characterization and functional analysis of recombinant human SNX12 in endosomal sorting"*
**作者**：Zhang L, Wang X, et al.
**摘要**：研究利用大肠杆菌系统表达重组SNX12蛋白，解析了其PX结构域与磷脂酰肌醇结合的分子机制，并证实其在细胞内膜运输和内体分选中的关键功能。
3. **文献名称**：*"Recombinant expression and purification of human SNX12: Role in cellular cholesterol homeostasis"*
**作者**：Lee S, Kim M, et al.
**摘要**：报道了重组SNX12蛋白在HEK293细胞中的高效表达及纯化方法，功能实验表明SNX12通过与脂筏相关蛋白互作参与细胞内胆固醇稳态调节。
4. **文献名称**：*"Functional interaction of SNX12 with the retromer complex in protein trafficking pathways"*
**作者**：Patel R, Brown K, et al.
**摘要**：通过重组SNX12蛋白的共沉淀实验，发现其与Retromer复合体的亚基VPS35直接结合，揭示了SNX12在逆向运输通路中协助货物蛋白分选的功能机制。
---
注：以上文献为模拟示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
Recombinant human SNX12 (Sorting Nexin 12) protein is a member of the sorting nexin family, which plays critical roles in intracellular membrane trafficking, cargo sorting, and endosomal dynamics. SNX12 contains a conserved Phox homology (PX) domain that binds phosphoinositides, enabling its recruitment to endosomal membranes. Unlike some sorting nexins, SNX12 lacks a Bin-Amphiphysin-Rvs (BAR) domain but may interact with other proteins to regulate vesicle formation and transport. It is implicated in retrograde trafficking from endosomes to the trans-Golgi network, influencing receptor recycling, lysosomal degradation, and cellular signaling pathways.
Studies suggest SNX12 participates in maintaining endosomal-lysosomal system integrity, with potential links to neurodegenerative diseases like Alzheimer’s and Parkinson’s, where disrupted trafficking contributes to pathology. Its expression is observed in multiple tissues, with notable roles in neuronal and immune cells. Recombinant SNX12 protein, typically produced in bacterial or mammalian expression systems, is used to study its structure, biochemical interactions, and mechanistic contributions to membrane dynamics. Applications include in vitro binding assays, functional studies with lipid membranes, and screening for therapeutic modulators targeting trafficking-related disorders. Further research aims to clarify its regulatory networks and disease associations.
×
