| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNRP70 |
| Uniprot No | P08621 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-210 aa |
| 活性数据 | MTQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERKRREKIERRQQEVETELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRH |
| 分子量 | 28.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SNRP70蛋白的3篇参考文献示例(文献信息为模拟内容，供参考)：
1. **文献名称**：Structural Insights into Human SNRP70 Protein in Spliceosome Assembly
**作者**：Li, X. et al.
**摘要**：该研究通过X射线晶体学解析了重组人SNRP70蛋白的结构，揭示了其与U1小核核糖核蛋白(snRNP)复合物中RNA的相互作用机制，为理解剪接体组装提供了分子基础。
2. **文献名称**：SNRP70 Knockdown Alters mRNA Splicing in Cancer Cells
**作者**：Wang, Y. et al.
**摘要**：文章利用重组SNRP70蛋白进行功能补偿实验，发现SNRP70缺失会导致乳腺癌细胞中异常剪接事件增多，提示其在维持RNA剪接保真性中的关键作用。
3. **文献名称**：Recombinant SNRP70 Production and Autoantibody Detection in Autoimmune Diseases
**作者**：Zhang, R. et al.
**摘要**：该研究通过大肠杆菌系统高效表达重组SNRP70蛋白，并验证其作为系统性红斑狼疮(SLE)患者血清自身抗体的特异性靶标，推动其临床诊断应用。
注：以上文献为模拟内容，实际引用时请以真实文献为准。建议通过PubMed或Web of Science检索关键词“SNRP70 recombinant”获取最新研究。
Small nuclear ribonucleoprotein 70 kDa (SNRP70) is a critical component of the spliceosome, a macromolecular complex responsible for pre-mRNA splicing in eukaryotic cells. As part of the U1 small nuclear ribonucleoprotein (snRNP) complex, SNRP70 plays a pivotal role in recognizing the 5' splice site of introns during spliceosome assembly. Structurally, it contains an N-terminal RNA recognition motif (RRM) that binds to U1 snRNA and a C-terminal glycine-rich domain involved in protein-protein interactions.
Recombinant human SNRP70 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, biologically active form for research applications. Its recombinant version enables studies on spliceosome dynamics, RNA processing mechanisms, and aberrations linked to diseases. Dysregulation of SNRP70 has been implicated in autoimmune disorders like systemic lupus erythematosus (SLE), where autoantibodies target spliceosomal components, as well as in cancers characterized by aberrant mRNA splicing.
Researchers utilize recombinant SNRP70 to investigate its structural interactions, screen therapeutic compounds, or model splicing defects. Its role in maintaining mRNA diversity underscores its importance in cellular function, disease pathology, and potential diagnostic or therapeutic targeting.
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