| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX11 |
| Uniprot No | Q9Y5W9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270 aa |
| 活性数据 | MGFWCRMSEN QEQEEVITVR VQDPRVQNEG SWNSYVDYKI FLHTNSKAFT AKTSCVRRRY REFVWLRKQL QRNAGLVPVP ELPGKSTFFG TSDEFIEKRR QGLQHFLEKV LQSVVLLSDS QLHLFLQSQL SVPEIEACVQ GRSTMTVSDA ILRYAMSNCG WAQEERQSSS HLAKGDQPKS CCFLPRSGRR SSPSPPPSEE KDHLEVWAPV VDSEVPSLES PTLPPLSSPL CCDFGRPKEG TSTLQSVRRA VGGDHAVPLD PGQLETVLEK |
| 分子量 | 30.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SNX11蛋白的3篇代表性文献(基于假设性内容整理，实际引用需核实具体论文)：
1. **文献名称**: **"SNX11 regulates synaptic vesicle recycling through its PX-BAR domain-mediated membrane interaction"**
**作者**: Lee JH, et al.
**摘要**: 本研究通过重组表达人SNX11蛋白，揭示了其PX-BAR结构域在突触小泡回收中的关键作用。实验表明SNX11通过与脂膜结合促进内吞过程，并可能调节神经递质释放。
2. **文献名称**: **"Characterization of SNX11 as a novel regulator of endosomal trafficking in human cells"**
**作者**: Zhang Y, et al.
**摘要**: 利用重组SNX11蛋白进行体外结合实验，发现其与网格蛋白复合物相互作用，调控早期内体到反式高尔基体的运输路径，提示SNX11在细胞内膜运输中的潜在调控机制。
3. **文献名称**: **"SNX11 deficiency impairs autophagosome-lysosome fusion and contributes to neurodegenerative pathology"**
**作者**: Wang R, et al.
**摘要**: 通过重组SNX11蛋白的功能恢复实验，发现SNX11缺失导致自噬体-溶酶体融合障碍，加剧帕金森病模型中的神经元损伤，表明其与神经退行性疾病的相关性。
4. **文献名称**: **"Crystal structure and biochemical analysis of the human SNX11 PX domain"**
**作者**: Chen L, et al.
**摘要**: 解析了重组SNX11蛋白的PX结构域晶体结构，揭示其特异性结合磷酸肌醇的分子基础，为设计针对SNX11功能异常疾病的干预策略提供依据。
**注意**：以上文献信息为模拟生成，实际研究中需通过PubMed/Google Scholar等数据库检索真实文献(可用关键词：SNX11 + recombinant/sorting nexin/membrane trafficking)。建议使用Zotero或EndNote管理文献信息并核对准确性。
SNX11 (Sorting Nexin 11) is a member of the sorting nexin family, a group of proteins characterized by their phosphoinositide-binding Phox homology (PX) domains, which facilitate membrane association and intracellular trafficking. As a peripheral membrane protein, SNX11 plays a critical role in endosomal sorting, vesicle formation, and protein trafficking between cellular compartments. It interacts with components of the endosomal sorting complex required for transport (ESCRT) machinery and regulates the lysosomal degradation pathway, particularly in the context of receptor downregulation and autophagic processes. Structurally, SNX11 contains a conserved PX domain at its N-terminus and a C-terminal region with coiled-coil motifs that mediate protein-protein interactions.
Emerging studies link SNX11 to various cellular functions, including neuronal development, immune response modulation, and cancer progression. Dysregulation of SNX11 has been implicated in pathologies such as neurodegenerative disorders and malignancies, likely due to its role in maintaining endosomal-lysosomal system integrity. Recombinant human SNX11 protein is widely used in biochemical assays, cell-based studies, and structural analyses to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it enables researchers to explore SNX11's interaction networks, phosphoinositide-binding specificity, and functional crosstalk with trafficking regulators. Its study provides insights into vesicle dynamics and potential therapeutic targets for trafficking-related diseases.
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