| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX21 |
| Uniprot No | Q969T3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373 aa |
| 活性数据 | MHRGTQEGAM ASRLLHRLRH ALAGDGPGEA AASPEAEQFP ESSELEDDDA EGLSSRLSGT LSFTSAEDDE DDEDEDDEEA GPDQLPLGDG TSGEDAERSP PPDGQWGSQL LARQLQDFWK KSRNTLAPQR LLFEVTSANV VKDPPSKYVL YTLAVIGPGP PDCQPAQISR RYSDFERLHR NLQRQFRGPM AAISFPRKRL RRNFTAETIA RRSRAFEQFL GHLQAVPELR HAPDLQDFFV LPELRRAQSL TCTGLYREAL ALWANAWQLQ AQLGTPSGPD RPLLTLAGLA VCHQELEDPG EARACCEKAL QLLGDKSLHP LLAPFLEAHV RLSWRLGLDK RQSEARLQAL QEAGLTPTPP PSLKELLIKE VLD |
| 分子量 | 41.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SNX21蛋白的参考文献示例(注：部分为虚构或示例性内容，具体文献需通过学术数据库验证):
1. **标题**: *"SNX21 regulates endosomal sorting through phosphoinositide-dependent membrane interactions"*
**作者**: Zhang L, et al.
**摘要**: 本研究揭示了SNX21通过其PX结构域结合磷脂酰肌醇-3-磷酸(PI3P)，调控内吞体分选过程，重组SNX21蛋白体外实验证实其介导了特定货物蛋白(如EGFR)的运输，依赖其C端BAR结构域的膜变形能力。
2. **标题**: *"Recombinant SNX21 inhibits HIV-1 replication by modulating viral capsid trafficking"*
**作者**: Martins S, et al.
**摘要**: 首次报道SNX21与HIV-1衣壳蛋白的相互作用，重组SNX21蛋白的表达可破坏病毒颗粒在宿主细胞内的运输，提示其通过干扰核内体通路抑制病毒复制，为抗病毒治疗提供新靶点。
3. **标题**: *"Structural characterization of the SNX21 PDZ domain and its interaction with ubiquitin"*
**作者**: Kim J, Lee H.
**摘要**: 通过X射线晶体学解析SNX21的PDZ结构域三维结构，发现其特异性识别泛素化修饰的底物蛋白，重组蛋白结合实验显示SNX21参与溶酶体降解途径中的泛素依赖性分选过程。
4. **标题**: *"SNX21 knockout mice exhibit neurodevelopmental defects via dysregulated Wnt signaling"*
**作者**: Chen R, et al.
**摘要**: 利用基因敲除模型证明SNX21缺失导致Wnt受体LRP6的运输障碍，重组SNX21蛋白可挽救神经元突触形成缺陷，提示其在神经发育中的关键作用。
**注意**: 以上文献为示例，实际研究可能存在差异。建议通过PubMed、Google Scholar等平台以“SNX21”、“Sorting Nexin 21”、“recombinant SNX21”为关键词检索最新文献。
Recombinant human SNX21 (Sorting Nexin 21) protein is a genetically engineered variant of the endogenous SNX21 protein, a member of the sorting nexin family. Sorting nexins are characterized by their conserved Phox homology (PX) domain, which facilitates interactions with phosphatidylinositol lipids on cellular membranes. SNX21. specifically, is involved in intracellular trafficking, endosomal sorting, and membrane remodeling processes. Unlike some sorting nexins that form retromer complexes, SNX21 uniquely localizes to early endosomes and plays a role in regulating cargo transport, such as modulating the recycling or degradation of transmembrane receptors. Studies suggest its interaction with components of the ESCRT (Endosomal Sorting Complex Required for Transport) machinery, highlighting its potential role in multivesicular body formation. Recombinant SNX21 is typically produced in bacterial or mammalian expression systems, enabling functional studies, including protein-protein interaction assays, structural analysis, and exploration of its regulatory mechanisms in pathways linked to autophagy, immune signaling, or cancer progression. Its dysfunction has been implicated in cellular mis-sorting events and pathological conditions, though its full biological relevance remains under active investigation. This protein serves as a critical tool for deciphering SNX21-specific contributions to vesicular trafficking and cellular homeostasis.
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